Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose^? Bead Conjugate)

• 产品编号：CST-4306S      品牌：CST       原厂货号：4306S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate) 兔单抗能检测内源性Thr183和Tyr185磷酸化的p46 、p54 SAPK/JNK蛋白水平。该抗体不能识别磷酸化的p44/42、p38 MAPK。该单克隆抗体是采用合成的与人员SAPK/JNK蛋白Thr183/Tyr185位点周围残基相一致的磷酸化肽段免疫动物而获得。通过氨基与羟基丁二酰亚胺 (NHS)活化的琼脂糖珠共价结合作用，抗体被固定在琼脂糖珠上。Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate)兔单抗可用于Thr183/Tyr185位点磷酸化的SAPK/JNK蛋白的免疫沉淀分析。预期此偶联抗体与未偶联的Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668具有相同的物种交叉反应。应激活化蛋白激酶/Jun氨基末端激酶SAPK/JNK能够有效、优先地被多种环境压力激活，这些环境压力包括紫外照射和gamma 辐射、神经酰胺、炎性因子，在某些情况下也包括生长因子和GPCR激动剂 (1-6)。正如其他的 MAPKs，核心信号传导单元包括一个MAPKKK，通常是MEKK1-MEKK4，或一种混合谱系酶 (MLKs），这些MAPKKK激酶能磷酸化并激活 MKK4/7。活化后，MKKs磷酸化并激活SAPK/JNK 激酶 (2)。应激反应信号经Rho 家族(Rac, Rho, cdc42)的小GTP酶被传递到这个级联信号反应中 (3)。Rac1 和 cdc42蛋白都能调节MEKKs、 MLKs的激活(3)。另外一种情况是，MKK4/7能够通过生发中心激酶 (GCK)家族成员的刺激，在一个非GTP酶依赖机制中被激活 (4)。有三种不同的SAPK/JNK基因，而每一种都会经过选择性剪切产生多种异构体(3)。活化的二聚体SAPK/JNK，能转位到细胞核中，通过它对 c-Jun, ATF-2和其他转录因子的效应而调节转录过程(3,5)。

1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
2. Ichijo, H. (1999) Oncogene 18, 6087-93.
3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

原厂资料：

Specificity / Sensitivity

Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of p46 and p54 SAPK/JNK only when phosphorylated at Thr183 and Tyr185. This antibody does not recognize phosphorylated p44/42 or p38 MAP kinases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK protein.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of SAPK/JNK phosphorylated at Thr183 and Tyr185. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668.

Background

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
2. Ichijo, H. (1999) Oncogene 18, 6087-93.
3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.