IRF-3 (D83B9) Rabbit mAb

• 产品编号：CST-4302S      品牌：CST       原厂货号：4302S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRF-3 (D83B9) Rabbit mAb能够检测内源性IRF-3蛋白总体水平。此单克隆抗体是通过合成人源对应的IRF-3 C-末端周围的肽段来免疫动物而获得。干扰素调控因子(IRFs) 包含一个家族的转录调控因子，这个家族主要是在 Jak/Stat信号通路中起作用，在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自身免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都有同源性。IRF家族成员通过与具有相似的DNA-结合区域的蛋白的反应而调控转录，例如IFN刺激的应答原件(ISRE), IFN 保守基序 (ICS) 和IFN 调控元件(IRF-E) (2)。IRF-3能抑制细胞的生长并在调控天然免疫应答基因中发挥了重要的作用(1-4)。在未受到刺激的细胞中, IRF-3 存在于细胞质中。病毒感染导致IRF-3磷酸化及其在细胞核的定位并激活具有IRF-3结合位点的启动子。IRF-3的磷酸化位点位于C-末端丝氨酸和苏氨酸簇中 (在385和405位点之间)导致了其与p300/CBP共激活蛋白的结合，从而促进DNA的结合和转录的活性(5)。在感染期间, IRF-3很可能是通过包括TLR和IKKε和 TBK1激酶复合体在内的一系列的信号通路而激活(6,7)。在受到病毒的感染、病毒核蛋白壳表达以及双链RNA的处理后，IRF-3在Ser396位点发生磷酸化，这些事件对IRF-3的激活非常重要(8)。

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Hiscott, J. et al. (1999) J Interferon Cytokine Res 19, 1-13.
4. Kim, T.Y. et al. (2003) J Biol Chem 278, 15272-8.
5. Yoneyama, M. et al. (2002) J Interferon Cytokine Res 22, 73-6.
6. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
7. Kopp, E. and Medzhitov, R. (2003) Curr Opin Immunol 15, 396-401.
8. Servant, M.J. et al. (2003) J Biol Chem 278, 9441-7.

原厂资料：

Specificity / Sensitivity

IRF-3 (D83B9) Rabbit mAb detects endogenous levels of total IRF-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human IRF-3.

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of carboxyl-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double stranded RNA treatment. These events likely play a role in activation of IRF-3 (8).

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Hiscott, J. et al. (1999) J Interferon Cytokine Res 19, 1-13.
4. Kim, T.Y. et al. (2003) J Biol Chem 278, 15272-8.
5. Yoneyama, M. et al. (2002) J Interferon Cytokine Res 22, 73-6.
6. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
7. Kopp, E. and Medzhitov, R. (2003) Curr Opin Immunol 15, 396-401.
8. Servant, M.J. et al. (2003) J Biol Chem 278, 9441-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody