PDAP1 Antibody

• 产品编号：CST-4300S      品牌：CST       原厂货号：4300S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PDAP1抗体可以识别内源性的总PDAP1蛋白。60KDa处可见非特异性条带。合成与人PDAP1蛋白的中心区域邻近氨基酸残基序列已知的肽段，免疫动物获得多克隆抗体。抗体由蛋白A和肽段亲和层析法纯化。血小板衍生的生长因子（PDGF）蛋白以二聚体的形式发挥功能（例如，PDGF AA, PDGF AB, PDGF BB, PDGF CC和PDGF DD），可以与受体酪氨酸激酶结合，激活细胞质内含有SH2结构域的蛋白以控制多种信号通路，发挥调控血管生成，细胞生长，肌动蛋白重构，迁移和分化的功能（1）。PDGFA-相关蛋白1（PDAP1）最早被鉴定为大鼠的视网膜癌细胞系中的一个新的PDGF相关蛋白（2）。当与PDGFA共纯化后，PDAP1与PDGFB结合性略微高于PDGFA（2）。尽管PDAP1的确切功能尚未可知，已发现可以促进PDGFA诱导的Swiss 3T3细胞的[3H]thymidine吸收和PDGFB生长因子下降的功能（2）。广泛表达的PDAP1在种属间高度保守（2,3），在体外可以被一些激酶磷酸化，包括PKC, PKA, CKI 和 CKII。其中CKII似乎是未受损伤的细胞中磷酸化PDAP1的最主要激酶（3）。

1. Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38.
2. Fischer, W.H. and Schubert, D. (1996) J Neurochem 66, 2213-6.
3. Shen, L. et al. (1996) Arch Biochem Biophys 327, 131-41.

原厂资料：

Specificity / Sensitivity

PDAP1 Antibody detects endogenous levels of total PDAP1 protein. Nonspecific bands are seen above 60 KDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the center of human PDAP1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Platelet derived growth factor (PDGF) proteins function as dimeric isoforms (i.e., PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind receptor tyrosine kinases and activate cytoplasmic SH2 domain-containing proteins to control multiple signaling pathways that regulate angiogenesis, cell growth, actin reorganization, migration and differentiation (1). PDGFA-associated protein 1 (PDAP1) was originally identified as a novel, PDGF-associated protein found in a rat retinal tumor cell line (2). While copurified with PDGFA, PDAP1 interacts with PDGFB at a slightly higher affinity than with PDGFA (2). Although the exact function of PDAP1 is unclear, it has been shown to both increase PDGFA-induced incorporation of [3H]thymidine in Swiss 3T3 cells and decrease PDGFB growth factor activity (2). Ubiquitously expressed PDAP1 is highly conserved among species (2,3) and is phosphorylated in vitro by several kinases, including PKC, PKA, CKI and CKII. Among this group, CKII seems to be the major kinase that phosphorylates PDAP1 in intact cells (3).

1. Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38.
2. Fischer, W.H. and Schubert, D. (1996) J Neurochem 66, 2213-6.
3. Shen, L. et al. (1996) Arch Biochem Biophys 327, 131-41.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.