PHLDA3 Antibody

• 产品编号：CST-4294S      品牌：CST       原厂货号：4294S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PHLDA3 Antibody能够检测内源性PHLDA3总蛋白。该多克隆抗体是由合成的人源PHLDA3蛋白N末端周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。Pleckstrin同源域家族A成员3 (PHLDA3)是三个相似的小蛋白的其中一个，三者有一个共同的pleckstrin同源(PH)域。PHLDA3和相关PHLDA1的表达发生在一些胎儿和成体组织中，这不同于PHLDA2在小鼠组织中更受限制的表达（1）。在顺铂处理的肿瘤细胞中，PHLDA3是被诱导表达的蛋白之一。顺铂是一种抗癌药，能够交联DNA，并通过激活肿瘤抑制基因p53促进细胞凋亡（2）。另外，在一项关于肿瘤发育过程中p53和Akt蛋白的反作用的研究中，证据表明PHLDA3通过p53参与促细胞凋亡。肿瘤抑制基因p53结合PHLDA3启动子诱导转录。被诱导的PHLDA3的过度表达增加细胞凋亡，而敲除PHLDA3 会使Akt的活性增加，并降低p53介导的细胞凋亡。PHLDA3的存在会与Akt的PH结构域的竞争，防止Akt的活化并促进Akt诱导的细胞存活通路（3）。

1. Frank, D. et al. (1999) Mamm Genome 10, 1150-9.
2. Kerley-Hamilton, J.S. et al. (2005) Oncogene 24, 6090-100.
3. Kawase, T. et al. (2009) Cell 136, 535-50.

原厂资料：

Specificity / Sensitivity

PHLDA3 Antibody detects endogenous level of total PHLDA3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PHLDA3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Pleckstrin homology-like domain family A member 3 (PHLDA3) is one of three relatively small, similar proteins that share a common pleckstrin homology (PH) domain. Expression of PHLDA3 and the related PHLDA1 occurs in several fetal and adult tissues, in contrast to the more restricted expression of PHLDA2 seen in mouse tissues (1). PHLDA3 is one of several proteins induced following treatment of tumor cells with cisplatin, an anti-cancer drug that cross-links DNA and promotes apoptosis through activation of the tumor suppressor p53 (2). Additional evidence that PHLDA3 is involved in promoting apoptosis through p53 came from a study examining the opposing effects of p53 and Akt in tumor development. Tumor suppressor p53 binds the PHLDA3 promoter to induce transcription. Induced overexpression of PHLDA3 increases apoptosis while deletion of PHLDA3 results in increased Akt activity and a reduction in p53-mediated apoptosis. PHLDA3 appears to compete with the PH domain of Akt, preventing Akt activation and promotion of Akt-induced cell survival pathways (3).

1. Frank, D. et al. (1999) Mamm Genome 10, 1150-9.
2. Kerley-Hamilton, J.S. et al. (2005) Oncogene 24, 6090-100.
3. Kawase, T. et al. (2009) Cell 136, 535-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody