K48-linkage Specific Polyubiquitin Antibody

• 产品编号：CST-4289S      品牌：CST       原厂货号：4289S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

K48-linkage Specific Polyubiquitin 抗体识别在Lys48链接的多泛素化链。这一抗体与单泛素和其他残基偶联的多泛素链无交叉反应。此抗体显示出与线性多泛素链轻微的交叉反应，与其他赖氨酸残基偶联的单泛素或多泛素链没有交叉反应。

Source / Purification

这一单抗是利用合成对应于人双泛素链的Lys48位分枝序列的肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。 Western blot analysis of recombinant monoubiquitin, K48-linked polyubiquitin, K48-linked polyubiquitin and K63-linked polyubiquitin using K48-linkage Specific Polyubiquitin Antibody (upper) and Ubiquitin (P4D1) Mouse mAb #3936 (lower). 使用K48-linkage Specific Polyubiquitin抗体（上）和泛素（P4D1）小鼠单克隆抗体＃3936（下）检测重组单泛素、K48-偶联多泛素和K63-偶联多泛素的免疫印迹分析结果。 Western blot analysis of seven distinct recombinant polyubiquitin chains using K48-linkage Specific Polyubiquitin Antibody (upper) and Ubiquitin (P4D1) Mouse mAb #3936 (lower). 使用K48-linkage Specific Polyubiquitin抗体（上）和泛素（P4D1）小鼠单克隆抗体＃3936（下）对七个不同的重组泛素链进行Western blot分析的结果。 Western blot analysis of L363 cells, untreated or treated with bortezomib as indicated, using K48-linkage Specific Polyubiquitin Antibody. 使用K48-linkage Specific Polyubiquitin抗体对经或未经bortezomib处理的L363细胞裂解物中该蛋白进行免疫印迹分析的结果。

原厂资料：

Specificity / Sensitivity

K48-linkage Specific Polyubiquitin 抗体识别在Lys48链接的多泛素化链。这一抗体与单泛素和其他残基偶联的多泛素链无交叉反应。此抗体显示出与线性多泛素链轻微的交叉反应，与其他赖氨酸残基偶联的单泛素或多泛素链没有交叉反应。

Source / Purification

这一单抗是利用合成对应于人双泛素链的Lys48位分枝序列的肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。 Western blot analysis of recombinant monoubiquitin, K48-linked polyubiquitin, K48-linked polyubiquitin and K63-linked polyubiquitin using K48-linkage Specific Polyubiquitin Antibody (upper) and Ubiquitin (P4D1) Mouse mAb #3936 (lower). 使用K48-linkage Specific Polyubiquitin抗体（上）和泛素（P4D1）小鼠单克隆抗体＃3936（下）检测重组单泛素、K48-偶联多泛素和K63-偶联多泛素的免疫印迹分析结果。 Western blot analysis of seven distinct recombinant polyubiquitin chains using K48-linkage Specific Polyubiquitin Antibody (upper) and Ubiquitin (P4D1) Mouse mAb #3936 (lower). 使用K48-linkage Specific Polyubiquitin抗体（上）和泛素（P4D1）小鼠单克隆抗体＃3936（下）对七个不同的重组泛素链进行Western blot分析的结果。 Western blot analysis of L363 cells, untreated or treated with bortezomib as indicated, using K48-linkage Specific Polyubiquitin Antibody. 使用K48-linkage Specific Polyubiquitin抗体对经或未经bortezomib处理的L363细胞裂解物中该蛋白进行免疫印迹分析的结果。

Background

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH₂ of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).

1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
3. Hochstrasser, M. (2000) Science 289, 563-4.
4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.
8. Komander, D. (2009) Biochem Soc Trans 37, 937-53.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody