MyD88 (D80F5) Rabbit mAb

• 产品编号：CST-4283S      品牌：CST       原厂货号：4283S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MyD88 (D80F5) Rabbit mAb 能够检测内源MyD88总蛋白水平。此单克隆抗体是通过合成人源对应的MyD88 Cys233位点周围的肽段来免疫动物而获得。Toll样受体(TLR) 家族成员, 是因与其密切相关的果蝇Toll受体而命名的，TLR在天然免疫中发挥了极其重要的作用(1-3)。TLRs 能够识别不同病原菌中保守的结构域并介导防御反应。TLR的触发导致了NF-κB信号通路的激活并因此调控下游的免疫和炎症基因的表达。TLRs与IL-1受体家族都包含一个大约200个氨基酸的保守区域—TIR结构域。受到激活后，TLRs 与细胞质中的含有TIR区域的一系列接头蛋白作用，包括MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule)。这些反应导致了IRAK1 和 IRAK4的招募和激活，并与TRAF6 形成复合体激活 TAK1 和 IKK。IKK的激活导致了正常情况下截留NF-κB在细胞质中而失去活性的IκB的降解。MyD88 最初是作为骨髓分化过程中重要的基因而被鉴别而来，它能够在IL-6的刺激下快速诱导并促进M1白血病细胞转变成巨噬细胞(4-6)。 此基因包含N-末端的死亡区域、C-末端的TIR区域并在TLR/IL-1受体信号通路中作为接头分子(7)。 MyD88的死亡区域介导其与IRAK复合体的相互作用并引起信号级联反应从而激活NF-κB (8,9)。

1. Akira, S. (2003) J Biol Chem 278, 38105-8.
2. Beutler, B. (2004) Nature 430, 257-63.
3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
11. Muzio, M. et al. (1997) Science 278, 1612-5.
12. Wesche, H. et al. (1997) Immunity 7, 837-47.
13. Suzuki, N. et al. (2002) Nature 416, 750-6.
14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.
15. Harroch, S. et al. (1995) Nucleic Acids Res 23, 3539-46.
16. Hardiman, G. et al. (1996) Oncogene 13, 2467-75.
17. Bonnert, T.P. et al. (1997) FEBS Lett 402, 81-4.
18. Medzhitov, R. et al. (1998) Mol Cell 2, 253-8.
19. Wesche, H. et al. (1997) Immunity 7, 837-47.
20. Muzio, M. et al. (1997) Science 278, 1612-5.

原厂资料：

Specificity / Sensitivity

MyD88 (D80F5) Rabbit mAb detects endogenous levels of total MyD88 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys233 of human MyD88 protein.

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm.MyD88 was originally isolated as a myeloid differentiation primary response gene that is rapidly induced upon IL-6 stimulated differentiation of M1 myeloleukemic cells into macrophages (4-6). It contains an amino-terminal death domain separated from a carboxyl-terminal TIR domain and functions as an adaptor in TLR/IL-1 receptor signaling (7). The death domain of MyD88 mediates interactions with the IRAK complex triggering a signaling cascade that includes the activation of NF-κB (8,9).

1. Akira, S. (2003) J Biol Chem 278, 38105-8.
2. Beutler, B. (2004) Nature 430, 257-63.
3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
11. Muzio, M. et al. (1997) Science 278, 1612-5.
12. Wesche, H. et al. (1997) Immunity 7, 837-47.
13. Suzuki, N. et al. (2002) Nature 416, 750-6.
14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.
15. Harroch, S. et al. (1995) Nucleic Acids Res 23, 3539-46.
16. Hardiman, G. et al. (1996) Oncogene 13, 2467-75.
17. Bonnert, T.P. et al. (1997) FEBS Lett 402, 81-4.
18. Medzhitov, R. et al. (1998) Mol Cell 2, 253-8.
19. Wesche, H. et al. (1997) Immunity 7, 837-47.
20. Muzio, M. et al. (1997) Science 278, 1612-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.