Cytochrome c (136F3) Rabbit mAb

• 产品编号：CST-4280S      品牌：CST       原厂货号：4280S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cytochrome c (136F3) Rabbit mAb 兔单抗能够检测内源水平细胞色素c总蛋白。该单克隆抗体是采用合成人源细胞色素C 67-78 位氨基酸相对应的肽段来免疫动物而获得。细胞色素C是一种保守的电子传递蛋白，作为呼吸链的组分存在于于线粒体内膜中(1)。受到凋亡刺激信号后，细胞色素C从线粒体释放， 并与procaspase-9 (47 kDa)/Apaf 1交联。此交联复合物促使caspase-9从没有活性的酶原转变成活化状态(2)。这一过程将进一步激发caspase-3的活化最终导致凋亡(3)。

1. Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
2. Li, P. et al. (1997) Cell 91, 479-489.
3. Liu, X. et al. (1996) Cell 86, 147-157.

原厂资料：

Specificity / Sensitivity

Cytochrome c (136F3) Rabbit mAb detects endogenous levels of total cytochrome c protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids 67-78 of human cytochrome c.

Background

Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).

1. Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
2. Li, P. et al. (1997) Cell 91, 479-489.
3. Liu, X. et al. (1996) Cell 86, 147-157.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.