Pan-Keratin (C11) Mouse mAb (Biotinylated)

• 产品编号：CST-4279S      品牌：CST       原厂货号：4279S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

Pan-Keratin (C11) Mouse mAb (生物素标记)识别内源性keratin 4、5、6、8、10、13和18总蛋白，但是不能与其它keratin蛋白发生交叉反应。通过来自A431细胞的一个细胞骨架的制备去免疫动物从而生成出单克隆抗体。Cell Signaling Technology的该抗体经优化后连接生物素。非连接的抗体Pan-Keratin (C11) Mouse mAb #4545可与人源、大鼠和猴pan-keratin蛋白发生反应。预测此抗体与Pan-Keratin (C11) Mouse mAb (生物素标记)也可识别这些物种的pan-keratin蛋白。

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

原厂资料：

Specificity / Sensitivity

Pan-Keratin (C11) Mouse mAb (Biotinylated) detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells.

Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Pan-Keratin (C11) Mouse mAb #4545 reacts with human, rat and monkey pan-keratin. CST expects that Pan-Keratin (C11) Mouse mAb (Biotinylated) will also recognize pan-keratin in these species.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot antibody