Phospho-Rb (Ser807/811) (D20B12) XP? Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-4277S      品牌：CST       原厂货号：4277S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

.Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit 单抗（偶联Alexa Fluor® 488）能够检测到在807，811位丝氨酸分别磷酸化或两个位点丝氨酸都经过磷酸化的内源性Rb蛋白。该抗体与608位丝氨酸磷酸化后的Rb蛋白没有交叉反应。单克隆抗体由合成肽段免疫动物产生，该肽段与人Rb蛋白807/811位丝氨酸附近氨基酸一致。Cell Signaling Technology公司抗体偶联Alexa Fluor® 488荧光素染料并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联Alexa Fluor® 488的Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit mAb#8516显示同样的物种交叉反应.成视网膜细胞瘤抑制蛋白，Rb，通过控制G1期的细胞限制点以调控细胞增殖（1）。Rb有3个功能迥异的结构域，与一系列重要的调控蛋白相结合，包括E2F家族调控因子，c-Abl酪氨酸激酶以及带有LXCXE保守结构域的蛋白（2-4）。CDK引起的细胞周期依赖性磷酸化会抑制Rb与靶蛋白结合从而促进细胞周期的进行（5）。细胞周期蛋白 D-CDK4/6起始的磷酸化，伴随着细胞周期蛋白E-CDK2的磷酸化，似乎能够失活Rb并引发细胞周期（6）。体外实验已经检测到有不同的细胞周期蛋白依赖性激酶/细胞周期蛋白复合物（6-8）同时体内试验证明细胞周期蛋白D1对于780位丝氨酸的磷酸化是必需的（9）。

1. Sherr, C.J. (1996) Science 274, 1672-7.
2. Nevins, J.R. (1992) Science 258, 424-9.
3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

原厂资料：

Specificity / Sensitivity

Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of Rb protein only when phosphorylated at Ser807, Ser811, or at both sites. This antibody does not cross-react with Rb phosphorylated at Ser608.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser807/811 of human Rb protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #8516.

Background

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

1. Sherr, C.J. (1996) Science 274, 1672-7.
2. Nevins, J.R. (1992) Science 258, 424-9.
3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze.