Fer (5D2) Mouse mAb

• 产品编号：CST-4268S      品牌：CST       原厂货号：4268S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Fer (5D2) Mouse mAb检测内源性Fer总蛋白。该抗体不能与其它相关蛋白质发生交叉反应。通过重组截短Fer蛋白去免疫动物从而制备出单克隆抗体。er蛋白是一个Fes/Fps家族非受体蛋白酪氨酸激酶。像许多其它细胞质酪氨酸激酶一样，Fer蛋白包含一个长的氨基末端区域、一个中间SH2结构域和一个羧基末端激酶区域。与细胞骨架蛋白相互作用一样，它的氨基末端区域是负责蛋白寡聚化。Fer蛋白在很多的细胞和组织类型中广泛表达，并且定位在细胞质膜和细胞核(1)。Fer蛋白酪通过生长因子和细胞因子能刺激氨酸激酶活性(2,3)。在激活之后，Fer蛋白能更进一步激活不同下游信号成分包括Stat3激酶(3)。Fer蛋白在调节细胞运动、肿瘤发生和炎症中起着重要作用(4)。

1. Hao, Q.L. et al. (1991) Mol Cell Biol 11, 1180-3.
2. Kim, L. and Wong, T.W. (1995) Mol Cell Biol 15, 4553-61.
3. Zoubeidi, A. et al. (2009) Mol Cancer Res 7, 142-55.
4. Greer, P. (2002) Nat Rev Mol Cell Biol 3, 278-89.

原厂资料：

Specificity / Sensitivity

Fer (5D2) Mouse mAb detects endogenous levels of total Fer protein. This antibody does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a truncated recombinant Fer protein.

Background

Fer is a nonreceptor protein-tyrosine kinase of the Fes/Fps family. Like many other cytoplasmic tyrosine kinases, Fer contains a long amino-terminal domain, a central SH2 domain, and a carboxy-terminal kinase domain. Its amino-terminal domain is responsible for protein oligomerization as well as interaction with cytoskeletal proteins. Fer is ubiquitously expressed in a wide variety of cell and tissue types, and is localized to both cytoplasm and nucleus (1). Tyrosine kinase activity of Fer can be stimulated by growth factors and cytokines (2,3). After activation, Fer can further activate various downstream signaling components including Stat3 (3). Fer plays an important role in regulation of cell movement, oncogenesis and inflammation (4).

1. Hao, Q.L. et al. (1991) Mol Cell Biol 11, 1180-3.
2. Kim, L. and Wong, T.W. (1995) Mol Cell Biol 15, 4553-61.
3. Zoubeidi, A. et al. (2009) Mol Cancer Res 7, 142-55.
4. Greer, P. (2002) Nat Rev Mol Cell Biol 3, 278-89.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody