Phospho-SRF (Ser103) Antibody

• 产品编号：CST-4261S      品牌：CST       原厂货号：4261S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SRF (Ser103) Antibody 兔多抗能检测内源性丝氨酸（103位点）磷酸化的SRF蛋白水平。该抗体能与大约52 kDa的非特异性蛋白发生交叉反应。该多克隆抗体通过使用与人源SRF蛋白丝氨酸（103位点）周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。血清效应因子(SRF) 是67 kDa的磷酸化蛋白，它与辅助因子一起修饰即时早期基因的转录，这些基因的启动子中包含血清效应元件(1,2)。SRF包含一些磷酸化位点(3)，但是磷酸化的功能性序列目前还不明确。一些生长因子和钙调节激酶，如p90RSK、CaM kinase IV，能磷酸化SRF蛋白的丝氨酸103位点(4,5)，SRF蛋白的丝氨酸103位点是MAPKAP激酶2的细胞核上靶点(6)。

1. Treisman, R. (1995) EMBO J. 14, 4905-4913.
2. Janknecht, R. et al. (1995) Carcinogenesis 16, 443-450.
3. Marais, R. M. et al. (1992) EMBO J. 11, 97-105.
4. Rivera, V. M. et al. (1993) Mol. Cell. Biol. 13, 6260-6273.
5. Miranti, C. K. et al. (1995) Mol. Cell. Biol. 15, 3672-3684.
6. Heidenreich, O. et al. (1999) JBC 274, 14434-14443.

原厂资料：

Specificity / Sensitivity

Phospho-SRF (Ser103) Antibody detects endogenous levels of SRF only when phosphorylated at serine 103. This antibody cross-reacts with a non-specific protein of approximately 52 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around serine 103 of human SRF. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The serum response factor (SRF) is a 67 kDa phospho-protein that, together with auxiliary factors, modulates transcription of immediate early genes containing serum response elements at their promoters (1,2). SRF contains several phosphorylation sites (3), but functional consequences of phosphorylation have not been identified unequivocally. Several growth factor- and calcium-regulated kinases, such as p90RSK and CaM kinase IV, can phosphorylate SRF at Ser103 (4,5), and Ser103 of SRF is also a nuclear target for MAPKAP kinase 2 (6).

1. Treisman, R. (1995) EMBO J. 14, 4905-4913.
2. Janknecht, R. et al. (1995) Carcinogenesis 16, 443-450.
3. Marais, R. M. et al. (1992) EMBO J. 11, 97-105.
4. Rivera, V. M. et al. (1993) Mol. Cell. Biol. 13, 6260-6273.
5. Miranti, C. K. et al. (1995) Mol. Cell. Biol. 15, 3672-3684.
6. Heidenreich, O. et al. (1999) JBC 274, 14434-14443.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.