GATA-6 (A549) Antibody

• 产品编号：CST-4253S      品牌：Cell Signaling Technology       原厂货号：4253S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GATA-6 (A549)抗体识别内源性的总GATA-6蛋白.多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源GATA-6蛋白Ala549的氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化 .GATA蛋白含有一组含有保守的锌指DNA结合结构域的转录因子，能够直接和核心元件GATA核苷酸序列结合(1-3)。共有六种脊椎动物GATA蛋白，GATA-1到GATA-6(3)。GATA-6对内胚层发育起关键作用（4）。它对心脏、肠和其它器官的发育所必需(5,6)。GATA-6的基因敲除会引起心脏导管的形成缺失，也不能发育胚外内胚层，这些会导致胚胎致死（4）。GATA-6的缺失表达或核内定位缺失明显存在于卵巢癌症中（7）。

1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
4. Cai, K.Q. et al. (2008) Dev Dyn 237, 2820-9.
5. Charron, F. and Nemer, M. (1999) Semin Cell Dev Biol 10, 85-91.
6. Haveri, H. et al. (2008) BMC Gastroenterol 8, 9.
7. Caslini, C. et al. (2006) Oncogene 25, 5446-61.

原厂资料：

Specificity / Sensitivity

GATA-6 (A549) Antibody detects endogenous levels of total GATA-6 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Ala549 of human GATA-6 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).GATA-6 plays a critical role in endoderm development (4). It is essential for development of the heart, gut and other organs (5,6). Knock out of GATA-6 is embryonic lethal due to defects in formation of the heart tube and a failure to develop extraembryonic endoderm (4). Loss of expression, or loss of nuclear localization of GATA-6 is apparent in a large number of ovarian tumors (7).

1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
4. Cai, K.Q. et al. (2008) Dev Dyn 237, 2820-9.
5. Charron, F. and Nemer, M. (1999) Semin Cell Dev Biol 10, 85-91.
6. Haveri, H. et al. (2008) BMC Gastroenterol 8, 9.
7. Caslini, C. et al. (2006) Oncogene 25, 5446-61.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at -20ºC. Do not alliquot the antibody