CHD4 Antibody

• 产品编号：CST-4245S      品牌：CST       原厂货号：4245S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CHD4 Antibody能够检测内源性的CHD4总蛋白水平。通过合成的与人源CHD4蛋白相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Chromodomain-helicase-DNA-binding domain (CHD)蛋白已经在多种生物中被鉴定(1,2)。由九个成员组成的蛋白家族已经被分为三个亚家族：subfamily I (CHD1和CHD2)、subfamily II (CHD3和CHD4)和subfamily III (CHD5、CHD6、CHD7、CHD8、CHD9)。所有CHD蛋白包含两个串联的N端chromodomains、一个SWI/SNF相关的ATPase结构域和一个C端DNA结合区域 (1,2)。chromodomains有助于结合到组蛋白的已甲基化赖氨酸残基，并且可与染色质的特异区域相互作用。SWI/SNF相关的ATPase区域可利用来自ATP水解的能量去修饰染色质结构。CHD蛋白经常发现在大的多蛋白复合物中，伴随着转录激活或抑制活性，这有该复合物中其它蛋白质监管。CHD3 (也称为Mi2-α)和CHD4 (也称为Mi2-β)是核小体重塑和组蛋白去乙酰化酶(NuRD)转录抑制复合物的中心成员，其包含HDAC1、HDAC2、RbAp48、RbAp46、MTA1、MTA2、MTA3和MBD3 (3-8)。CHD3和CHD4包含两个PHD (plant homeodomain)锌指结构，该结构直接结合到HDAC1和HDAC2。

1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
3. Tong, J.K. et al. (1998) Nature 395, 917-21.
4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
5. Zhang, Y. et al. (1998) Cell 95, 279-89.
6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
8. Fujita, N. et al. (2003) Cell 113, 207-19.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Horse

Specificity / Sensitivity

CHD4 Antibody detects endogenous levels of total CHD4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CHD4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multi-protein complexes, with their transcriptional activation or repression activity governed by other proteins also found in the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RbAp48, RbAp46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2.

1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
3. Tong, J.K. et al. (1998) Nature 395, 917-21.
4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
5. Zhang, Y. et al. (1998) Cell 95, 279-89.
6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
8. Fujita, N. et al. (2003) Cell 113, 207-19.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.