nNOS Antibody

• 产品编号：CST-4234S      品牌：CST       原厂货号：4234S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

nNOS Antibody 兔多抗识别内源性的nNOS总蛋白。该多克隆抗体由合成的人类nNOS蛋白氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。一氧化氮合酶（NOS）利用L-精氨酸、氧气和辅助因子催化产生一氧化氮（NO）和瓜氨酸。其三个家庭成员已被报道：主要在神经组织中发现的神经元型NOS（nNOS），在肾脏和心血管系统中被γ干扰素和脂多糖诱导的诱导型NOS（iNOS），以及血管中表达的内皮一氧化氮合酶（eNOS）（1）。一氧化氮是一种信使分子，与全身不同功能有关，包括维持血管的完整性，动态平衡，突触可塑性，长时程增强，学习和记忆（2,3）。nNOS选择性的结合PSD-95的第二个PDZ结构域，nNOS的酶活性可以被CaM-KII磷酸化Ser847或者NMDA受体通道介导的钙离子内流所下调（4-5）。

1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
2. Son, H. et al. (1996) Cell 87, 1015-10123.
3. Hawkins, R.D. (1996) Neuron 16, 465-467.
4. Watanabe, Y. et al. (2003) Biochem J 372, 465-71.
5. Sears, C.E. et al. (2004) Philos Trans R Soc Lond B Biol Sci 359, 1021-44.

原厂资料：

Specificity / Sensitivity

nNOS Antibody detects endogenous levels of total nNOS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human nNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nitric Oxide Synthase (NOS) catalyses the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).nNOS binds selectively to the second PDZ domain of PSD-95, and the enzymatic activity of nNOS is either downregulated by phosphorylation at Ser847 by CaM-KII or stimulated by calcium influxes governed by the NMDA receptor channels (4-5).

1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
2. Son, H. et al. (1996) Cell 87, 1015-10123.
3. Hawkins, R.D. (1996) Neuron 16, 465-467.
4. Watanabe, Y. et al. (2003) Biochem J 372, 465-71.
5. Sears, C.E. et al. (2004) Philos Trans R Soc Lond B Biol Sci 359, 1021-44.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody