Fas (C18C12) Rabbit mAb

• 产品编号：CST-4233S      品牌：CST       原厂货号：4233S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Fas (C18C12) Rabbit mAb兔单抗能够检测内源性水平的人源Fas总蛋白。该单克隆抗体是采用合成的人源Fas蛋白胞内区赖氨酸(259位)周围残基相对应的肽段免疫动物而制备的。受体Fas及其配体FasL的结合会触发细胞凋亡通路，这在免疫调节、发育和癌症进程中起着重要作用（1,2）。无论是Fas（ lpr小鼠）还是FasL（gld小鼠）的功能突变缺失都会导致淋巴结肿大和脾肿大，并减弱CD4-CD8-T淋巴细胞的细胞凋亡（3,4）。FasL (CD95L, Apo-1L)是II型跨膜蛋白，有280个氨基酸（糖基化后电泳条带约40 kDa），属于TNF家族，该家族还包括TNF-α，TRAIL和TWEAK。FasL蛋白与其受体结合，触发形成死亡诱导信号复合物（DISC），同时募集接头蛋白FADD和caspase-8（5）。在这个复合物中caspase-8的激活会引发半胱天冬酶级联反应，从而引起caspase-3的活化和随后的蛋白质裂解，最后导致细胞凋亡。与Fas不同，其在各种类型细胞中都会组成型表达，FasL主要在活化的T淋巴细胞、NK细胞和免疫优先位点表达（6）。FasL也作为一种逃避免疫监视的机制在几种不同类型的肿瘤中表达（7）。与TNF家族的其他成员类似， FasL可以被金属蛋白酶裂解产生可溶形式的26 kDa的三聚体（8,9）。

1. Suda, T. et al. (1993) Cell 75, 1169-78.
2. Lee, H.O. and Ferguson, T.A. (2003) Cytokine Growth Factor Rev 14, 325-35.
3. Watanabe-Fukunaga, R. et al. (1992) Nature 356, 314-7.
4. Hahne, M. et al. (1995) Int Immunol 7, 1381-6.
5. Nagata, S. (1997) Cell 88, 355-65.
6. Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2, 917-24.
7. Walker, P.R. et al. (1997) J Immunol 158, 4521-4.
8. Kayagaki, N. et al. (1995) J Exp Med 182, 1777-83.
9. Tanaka, M. et al. (1995) EMBO J 14, 1129-35.

原厂资料：

Specificity / Sensitivity

Fas (C18C12) Rabbit mAb detects endogenous levels of total human Fas protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys259 of human Fas, within the intracellular region.

Background

Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9).

1. Suda, T. et al. (1993) Cell 75, 1169-78.
2. Lee, H.O. and Ferguson, T.A. (2003) Cytokine Growth Factor Rev 14, 325-35.
3. Watanabe-Fukunaga, R. et al. (1992) Nature 356, 314-7.
4. Hahne, M. et al. (1995) Int Immunol 7, 1381-6.
5. Nagata, S. (1997) Cell 88, 355-65.
6. Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2, 917-24.
7. Walker, P.R. et al. (1997) J Immunol 158, 4521-4.
8. Kayagaki, N. et al. (1995) J Exp Med 182, 1777-83.
9. Tanaka, M. et al. (1995) EMBO J 14, 1129-35.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.