Phospho-Tie2 (Ser1119) Antibody

• 产品编号：CST-4226S      品牌：CST       原厂货号：4226S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化Tie2 (Ser1119) Antibody 仅在Tie2蛋白serin1119位点磷酸化时能检测到转染表达的Tie2的水平。多克隆抗体通过用多肽免疫动物得到，该磷酸化多肽是根据人的Tie2蛋白Ser1119附近的氨基酸序列合成的。多克隆抗体经过protein A和亲和层析纯化。Tie2/Tek 是一个几乎仅在内皮细胞上表达的酪氨酸激酶受体 (RTK)。它对于血管生成十分关键，对成年人血管内皮细胞的存活、完整性以及肿瘤血管新生都十分重要(1-3)。血管生成素（angiopoietins）家族的蛋白能作为配体和Tie2结合。有趣的是，这些配体具有相反的作用，比如Angiopoietin-1 (Ang1) 和 Angiopoietin-4 (Ang4)能刺激Tie2酪氨酸位点的磷酸化， 而Angiopoietin-2 (Ang2) 和Angiopoietin-3 (Ang3) 能抑制这种磷酸化(4,5)。下游的信号组分，包括Grb2, Grb7, Grb14, Shp2, PI3K激酶的p85亚基和p56/Dok-2通过它们的SH2或PTB结构域以酪氨酸磷酸化依赖的形式和Tie2相互作用(6,7)。Tyr992定位在Tie2分子的假定活化环状区是一个主要的自磷酸化位点(8)。Tie2羧基端Ser1119位点磷酸化会导致Tie2激酶活性增加(与杜克大学的 Chris Kontos博士个人交流所得)。

1. Ward, N.L. and Dumont, D.J. (2002) Semin. Cell Dev. Biol. 13, 19-27.
2. Jones, N. and Dumont, D.J. (2000) Cancer Metastasis Rev. 19, 13-17.
3. Partanen, J. and Dumont, D.J. (1999) Curr. Top. Microbiol. Immunol. 237, 159-172.
4. Ellis, L. M. et al. (2002) Oncology 16, 31-35.
5. Koh, G. Y. et al. (2002) Exp. Mol. Med. 34, 1-11.
6. Jones, N. et al. (1999) J. Biol. Chem. 274, 30896-30905.
7. Jones, N. et al. (2003) Mol. Cell. Biol. 23, 2658-2668.
8. Murray, B. W. et al. (2001) Biochem. 40, 10243-10253.

原厂资料：

Specificity / Sensitivity

Phospho-Tie2 (Ser1119) Antibody detects transfected levels of Tie2 protein only when phosphorylated at serine 1119.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1119 of human Tie2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells. It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3). A family of ligands known as the angiopoietins binds to Tie2. Interestingly, these ligands appear to have opposing actions, as Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2, whereas Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4,5). Downstream signaling components, including Grb2, Grb7, Grb14, Shp2, the p85 subunit of phosphatidylinositol 3-kinase and p56/Dok-2, interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains (6,7). Tyr992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8).Phosphorylation of Ser1119 at the carboxy-terminus of Tie2 results in an increase of Tie2 kinase activity (personal communication with Dr. Chris Kontos, Duke University).

1. Ward, N.L. and Dumont, D.J. (2002) Semin. Cell Dev. Biol. 13, 19-27.
2. Jones, N. and Dumont, D.J. (2000) Cancer Metastasis Rev. 19, 13-17.
3. Partanen, J. and Dumont, D.J. (1999) Curr. Top. Microbiol. Immunol. 237, 159-172.
4. Ellis, L. M. et al. (2002) Oncology 16, 31-35.
5. Koh, G. Y. et al. (2002) Exp. Mol. Med. 34, 1-11.
6. Jones, N. et al. (1999) J. Biol. Chem. 274, 30896-30905.
7. Jones, N. et al. (2003) Mol. Cell. Biol. 23, 2658-2668.
8. Murray, B. W. et al. (2001) Biochem. 40, 10243-10253.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.