CDC37 (D28H7) Rabbit mAb

• 产品编号：CST-4222S      品牌：CST       原厂货号：4222S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDC37 (D28H7) Rabbit mAb检测内源性CDC37总蛋白。通过人工合成人源CDC37蛋白Val367位点周围相应的多肽片段去免疫动物从而制备单克隆抗体。CDC37蛋白是一个HSP90伴侣复合物的重要组成成员(1,2)。该蛋白起初被证明涉及细胞周期进程，之后被发现有个广泛的作用，其作为一个很多激酶和其它蛋白质的分子伴侣 (1-3)。CDC37蛋白有一个氨基末端激酶结合区域，紧接着一个中央HSP90结合区域。通过保护新合成激酶多肽链避免降解和促进蛋白质成熟的接下来步骤进行，该蛋白在HSP90 复合物中招募和稳定激酶(4,5)。CDC37也抑制HSP90蛋白的活性，因此导致在复合物中构象改变，这将阻止靶激酶结合(6)。CDC37蛋白已被建议作为治疗靶点，因为在多种激酶通路涉及到增殖和癌细胞存活包括Raf、Akt、Src和ErbB2通路中起着重要作用(7,8)。

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22.
2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92.
3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7.
4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28.
5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9.
6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9.
7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85.
8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.

原厂资料：

Specificity / Sensitivity

CDC37 (D28H7) Rabbit mAb detects endogenous levels of total CDC37 protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val367 of human CDC37 protein. 

Background

CDC37 is an important component of the HSP90 chaperone complex (1,2). It was initially identified for its involvement in cell-cycle progression and was later found to have a much broader role as a chaperone for a wide variety of kinases and other proteins (1-3). CDC37 protein has an amino-terminal kinase binding domain followed by a central HSP90 binding domain. It recruits and stabilizes kinases in the HSP90 complex by protecting the newly synthesized kinase peptide chain from degradation and promoting the next step of protein maturation (4,5). CDC37 also suppresses the ATPase activity of HSP90, thereby leading to conformational changes in the complex that preclude target kinase loading (6). CDC37 has been proposed as a therapeutic target because of its important role in multiple kinase pathways involved in proliferation and cancer cell survival, including Raf, Akt, Src, and ErbB2 pathways (7,8). CDC37蛋白是一个HSP90伴侣复合物的重要组成成员(1,2)。

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22.
2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92.
3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7.
4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28.
5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9.
6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9.
7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85.
8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody