Phospho-NMDA Receptor 2B (GluN2B) (Tyr1472) Antibody

• 产品编号：CST-4208S      品牌：CST       原厂货号：4208S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-NMDAR2B (Tyr1472) Antibody 兔多抗识别内源性的Tyr1472磷酸化的NMDAR2B蛋白。该多克隆抗体由合成的人类NMDAR2B蛋白Tyr1472位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。N-甲基-D-天冬氨酸（NMDA）受体形成至少有一个NR1和一个NR2A-D亚基的异二聚体。其各种在脑内分布和功能特性不同的亚型是由NR1的选择性剪切和NR2的差异表达所形成。NR1亚基结合的共刺激激动剂甘氨酸，NR2结合的神经递质谷氨酸。NMDA受体激活或离子通道的开放，允许Na+和Ca2+离子进入细胞和K+流出细胞（1）。每个亚基有一个细胞质域，可直接通过蛋白激酶/磷酸酶进行修饰调控（2）。蛋白激酶C可以在Ser890/Ser896磷酸化NR1亚基（NMDAR1），PKA可以磷酸化NR1的Ser897（3）。NR1的PKC磷酸化降低其对钙调蛋白的亲和力，从而防止钙调蛋白对NMDA受体的抑制作用（4）。PKA磷酸化NR1可能可抵消钙调蛋白对受体的抑制作用（5）。NMDA受体介导长时程增强和慢速突触后兴奋，从而在学习，神经发育和神经可塑性中发挥中心作用（6）。EphrinB2结合EphB受体，导致Src家族络氨酸激酶的激活，从而磷酸化NMDAR2B的Tyr1252, Tyr1336和Tyr1472位点。磷酸化的NMDAR2B增强NMDA受体在接受谷氨酸能刺激时调节Ca2+内流的能力（7,8）。NMDAR2B的Tyr1472磷酸化位点由CST公司使用PhosphoScan®和CST的磷酸化位点质谱平台发现。NMDAR2B在Tyr1472上的磷酸化在缺血大鼠脑组织分离提取物中可见。更多信息，请参见PhosphoSitePlus®，CST修饰位点知识库，www.phosphosite.org。

1. Liu, X.B. et al. (2004) J Neurosci 24, 8885-95.
2. Westphal, R.S. et al. (1999) Science 285, 93-6.
3. Tingley, W.G. et al. (1997) J Biol Chem 272, 5157-66.
4. Hisatsune, C. et al. (1997) J Biol Chem 272, 20805-10.
5. Raman, I.M. et al. (1996) Neuron 16, 415-21.
6. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.
7. Nakazawa, T. et al. (2001) J Biol Chem 276, 693-9.
8. Takasu, M.A. et al. (2002) Science 295, 491-5.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse

Specificity / Sensitivity

Phospho-NMDAR2B (Tyr1472) Antibody detects endogenous levels of NMDAR2B only when phosphorylated at Tyr1472.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1472 of NMDAR2B. Antibodies are purified by protein A and peptide affinity chromatography.

Background

N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).EphrinB2 binding to the receptor EphB leads to the activation of Src family tyrosine kinases, which phosphorylate NMDAR2B at Tyr1252, Tyr1336 and Tyr1472. In turn, phosphorylated NMDAR2B enhances the ability of the functional NMDA receptor to regulate Ca2+ influx in response to glutamate (7, 8). Phosphorylation of NMDAR2B at Tyr1472 was independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of NMDAR2B at Tyr1472 was observed in extracts isolated from ischemic rat brain. For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org.

1. Liu, X.B. et al. (2004) J Neurosci 24, 8885-95.
2. Westphal, R.S. et al. (1999) Science 285, 93-6.
3. Tingley, W.G. et al. (1997) J Biol Chem 272, 5157-66.
4. Hisatsune, C. et al. (1997) J Biol Chem 272, 20805-10.
5. Raman, I.M. et al. (1996) Neuron 16, 415-21.
6. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.
7. Nakazawa, T. et al. (2001) J Biol Chem 276, 693-9.
8. Takasu, M.A. et al. (2002) Science 295, 491-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.