Rig-I (D33H10) Rabbit mAb

• 产品编号：CST-4200S      品牌：CST       原厂货号：4200S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rig-I (D33H10) Rabbit mAb兔单抗能够检测内源Rig-I蛋白总体水平。此单克隆抗体是通过合成人源对应的Rig-I Gly719位点周围的肽段来免疫动物而获得。天然免疫抗病毒依赖Toll样受体(TLR)家族和RNA螺旋家族中的病毒探测蛋白诱导的几条平行的信号通路的相互结合，如 Rig-I (类维生素A酸诱导的基因I) 和 MDA-5 (黑素瘤分化相关的抗原5)，它们能够促进 I 型干扰素和抗病毒酶的转录(1-3)。TLRs 和解旋酶蛋白包含识别不同病毒分子模式的位点，包括DNA、单链RNA (ssRNA)、双链RNA (dsRNA)和糖蛋白。这些抗病毒的蛋白存在于细胞的不同区域中：TLRs (i.e. TLR3, TLR7, TLR8 和 TLR9) 是在红细胞浆质膜，解旋酶定位在细胞质中。Rig-I的表达受到维生素A酸、LPS、IFN 和病毒感染的诱导 (4,5)。Rig-I和MDA-5 都包含一个检测病毒双链RNA的DExD/H盒解旋区域和两个激活下游信号通路的N-末端的caspase招募区域(CARD)(4-7)。 Rig-I能够结合到双链RNA和病毒的末端的单链RNA的5'-三磷酸上，此5'-三磷酸在寄主RNA中不存在(8,9)。虽然结构上相关，Rig-I和MDA-5能够检测不同种类的病毒(10,11)。解旋酶的CARD区域足够引起信号和IFN的产生，同时被招募到MAVS/VISA/Cardif/IPS-1线粒体蛋白的CARD区域,并引起NF-κB、TBK1/IKKε 和 IRF-3/IRF-7 的激活(12-15)。

1. Yoneyama, M. and Fujita, T. (2007) J Biol Chem 282, 15315-8.
2. Meylan, E. and Tschopp, J. (2006) Mol Cell 22, 561-9.
3. Thompson, A.J. and Locarnini, S.A. (2007) Immunol Cell Biol 85, 435-45.
4. Imaizumi, T. et al. (2002) Biochem Biophys Res Commun 292, 274-9.
5. Zhang, X. et al. (2000) Microb Pathog 28, 267-78.
6. Yoneyama, M. et al. (2005) J Immunol 175, 2851-8.
7. Yoneyama, M. et al. (2004) Nat Immunol 5, 730-7.
8. Hornung, V. et al. (2006) Science 314, 994-7.
9. Pichlmair, A. et al. (2006) Science 314, 997-1001.
10. Kato, H. et al. (2006) Nature 441, 101-5.
11. Childs, K. et al. (2007) Virology 359, 190-200.
12. Meylan, E. et al. (2005) Nature 437, 1167-72.
13. Xu, L.G. et al. (2005) Mol Cell 19, 727-40.
14. Kawai, T. et al. (2005) Nat Immunol 6, 981-8.
15. Seth, R.B. et al. (2005) Cell 122, 669-82.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Pig

Specificity / Sensitivity

Rig-I (D33H10) Rabbit mAb detects endogenous levels of total Rig-I protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly719 of human Rig-I.

Background

Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5'-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1/IKKε, and IRF-3/IRF-7 (12-15).

1. Yoneyama, M. and Fujita, T. (2007) J Biol Chem 282, 15315-8.
2. Meylan, E. and Tschopp, J. (2006) Mol Cell 22, 561-9.
3. Thompson, A.J. and Locarnini, S.A. (2007) Immunol Cell Biol 85, 435-45.
4. Imaizumi, T. et al. (2002) Biochem Biophys Res Commun 292, 274-9.
5. Zhang, X. et al. (2000) Microb Pathog 28, 267-78.
6. Yoneyama, M. et al. (2005) J Immunol 175, 2851-8.
7. Yoneyama, M. et al. (2004) Nat Immunol 5, 730-7.
8. Hornung, V. et al. (2006) Science 314, 994-7.
9. Pichlmair, A. et al. (2006) Science 314, 997-1001.
10. Kato, H. et al. (2006) Nature 441, 101-5.
11. Childs, K. et al. (2007) Virology 359, 190-200.
12. Meylan, E. et al. (2005) Nature 437, 1167-72.
13. Xu, L.G. et al. (2005) Mol Cell 19, 727-40.
14. Kawai, T. et al. (2005) Nat Immunol 6, 981-8.
15. Seth, R.B. et al. (2005) Cell 122, 669-82.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.