IL-2Rβ Antibody

• 产品编号：CST-4198S      品牌：CST       原厂货号：4198S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IL-2Rβ Antibody能够检测内源水平IL-2Rβ蛋白总体水平。此多克隆抗体是通过合成人源对应的IL-2Rβ Thr420位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。IL-2是T细胞刺激细胞因子，因其对T细胞和NK细胞增殖的诱导和激活而知名(1,2)。IL-2也能促进外周Treg细胞的发育(3,4)。相反的，IL-2也参与到了激活对细胞死亡(AICD)的诱导中，此过程在后T细胞的扩充以增加 CD4+ T 细胞的Fas水平(5)。IL-2的效应是通过一个三聚体受体而介导，此三聚体包含IL-2Rα, IL-2Rβ和共有的γ链-γc (1,2)。 IL-2Rα特异性的以较低亲和度与 IL-2结合，而包括IL-2Rβ和γc亚单元的受体复合体结合亲和度明显提高。IL-15 也能结合到IL-2Rβ上 (1,2)。共有的γc链也被其它的细胞因子所利用，包括IL-4, IL-7, IL-9, IL-15和IL-21(1,2)。IL-2的结合起始了信号的级联反应，包括 Jak1, Jak3, Stat5和PI3K/Akt信号通路(1,2)。

1. Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.
2. Gaffen, S.L. and Liu, K.D. (2004) Cytokine 28, 109-23.
3. Fehérvari, Z. et al. (2006) Trends Immunol 27, 109-11.
4. Antony, P.A. et al. (2006) J Immunol 176, 5255-66.
5. Jaleco, S. et al. (2003) J Immunol 171, 61-8.

原厂资料：

Specificity / Sensitivity

IL-2Rβ Antibody detects transfected levels of total IL-2Rβ protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr420 of human IL-2Rβ. Antibodies are purified by protein A and peptide affinity chromatography.

Background

IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of Treg cells (3,4). Conversely, IL-2 is involved in activation induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). The common γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5 and the PI3K/Akt pathways (1,2).

1. Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.
2. Gaffen, S.L. and Liu, K.D. (2004) Cytokine 28, 109-23.
3. Fehérvari, Z. et al. (2006) Trends Immunol 27, 109-11.
4. Antony, P.A. et al. (2006) J Immunol 176, 5255-66.
5. Jaleco, S. et al. (2003) J Immunol 171, 61-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.