NAC1 Antibody (Rodent Preferred)

• 产品编号：CST-4183S      品牌：CST       原厂货号：4183S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NAC1抗体（啮齿类样品优选）识别大鼠和小鼠内源性的总NAC1 蛋白，而识别人NAC1 蛋白较弱.多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源NAC1蛋白的Ala321氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化 .NAC1或阿肯伯氏核是一种核内因子，属于POZ/BTB(Pox 病毒和锌指/ bric-a-brac tramtrack broad 复合体)。也叫BTBD14B，起初在单一的神经元前脑结构中被发现，并负责更新动机和成瘾行为(1,2)。NAC1招募HDAC3和HDAC4转录上抑制基因表达于神经元内(3)。并在中枢神经系统中特异共抑制其他POZ/BTB蛋白(4)。NAC1在一些肿瘤类型中上调，包括乳腺，肾脏细胞，肝癌细胞还有高分级的浆液性卵巢癌，所以长期以来，NAC1被认为是化疗抗性基因(5,6)。上述化疗耐受机制的报道是，通过NAC1负向调控GADD45通路(7)。NAC1在多潜能细胞中已被描述是由Oct-4, Sox2, Nanog, Sall1, KLF4 和Sall4组成的调控网络的一部分(8).

1. Kalivas, P.W. et al. (1999) Synapse 33, 153-9.
2. Mackler, S.A. et al. (2000) J Neurosci 20, 6210-7.
3. Korutla, L. et al. (2005) J Neurochem 94, 786-93.
4. Korutla, L. et al. (2009) Neurochem Int 54, 245-52.
5. Nakayama, K. et al. (2006) Proc Natl Acad Sci USA 103, 18739-44.
6. Yeasmin, S. et al. (2008) Clin Cancer Res 14, 1686-91.
7. Jinawath, N. et al. (2009) Oncogene 28, 1941-8.
8. Kim, J. et al. (2008) Cell 132, 1049-61.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

NAC1 Antibody (Rodent Preferred) detects endogenous levels of total NAC1 protein in mouse and rat and weakly detects human NAC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino acid sequence surrounding Ala321 of human NAC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

NAC1 or nuclear accumbens-1 is a nuclear factor that belongs to the POZ/BTB (Pox virus and zinc finger/bric-a-brac tramtrack broad complex) domain family. Also known as BTBD14B, it was originally identified in a unique neuronal forebrain structure responsible for reward motivation and addictive behaviors (1,2). NAC1 recruits HDAC3 and HDAC4 to transcriptionally repress gene expression in neuronal cells (3) and specifically co-represses other POZ/BTB proteins in the central nervous system (4). NAC1 is upregulated in several tumor types, including breast, renal cell, and hepatocellular carcinoma, as well as high grade ovarian serous carcinoma, where it has long been suspected as a chemoresistance gene (5,6). The chemoresistance mechanism reportedly occurs through NAC1 negative regulation of the GADD45 pathway (7). NAC1 has also been described as part of the extended transcriptional network in pluripotent cells that involves Oct-4, Sox2, Nanog, Sall1, KLF4 and Sall4 (8).

1. Kalivas, P.W. et al. (1999) Synapse 33, 153-9.
2. Mackler, S.A. et al. (2000) J Neurosci 20, 6210-7.
3. Korutla, L. et al. (2005) J Neurochem 94, 786-93.
4. Korutla, L. et al. (2009) Neurochem Int 54, 245-52.
5. Nakayama, K. et al. (2006) Proc Natl Acad Sci USA 103, 18739-44.
6. Yeasmin, S. et al. (2008) Clin Cancer Res 14, 1686-91.
7. Jinawath, N. et al. (2009) Oncogene 28, 1941-8.
8. Kim, J. et al. (2008) Cell 132, 1049-61.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody