Pim-3 (D17C9) Rabbit mAb

• 产品编号：CST-4165S      品牌：CST       原厂货号：4165S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Pim-3 (D17C9) Rabbit mAb兔单抗能够检测内源Pim-3蛋白总体水平。此单克隆抗体是通过合成人源对应的Pim-3 Ser275位点周围的肽段来免疫动物而获得。im 家族的蛋白包括Pim-1, Pim-2和Pim-3，它们是致癌基因编码的丝氨酸/苏氨酸激酶 (1). Pim-1 丝氨酸/苏氨酸激酶在造血细胞中高表达，在促有丝分裂信号传导中发挥了重要的作用，并且能够快速的被多种细胞生长因子诱导 (1-4)。 Pim-1 与 c-Myc相互协作而作用在淋巴细胞转变过程中，能够保护细胞远离生长因子消退和毒素诱导的细胞凋亡(5,6)。 Pim-1 通过直接介导c-Myb的DNA结合区域和转录因子共激活因子p100的的磷酸化而增强c-Myb的转录活性 (7,8)。Pim-1基因的超活性突变体在弥漫性大B细胞淋巴瘤中被发现(9)。 受到IL-6的诱导，Etk将Pim-1的Tyr218 位点磷酸化并增强Pim-1 的活性 (10)。 不同的Pim底物已经被鉴定，Bad 能被 Pim-1 和 Pim-2在 Ser112 位点磷酸化，这个位点的磷酸化改变了 Bad-诱导的细胞凋亡(11,12)。Pim-3 蛋白最初被发现在PC-12细胞中经去极化诱导并被命名为 Kid-1(13)。与其它的家族成员一样, Pim-3能磷酸化 Bad并抑制 Bad介导的凋亡(14)。Pim-3在很多种类的癌症中突变表达,导致了肺癌，胰腺癌, 结肠癌和胃癌(14-17)。

1. Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15.
2. Selten, G. et al. (1986) Cell 46, 603-11.
3. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12.
4. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20.
5. Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
6. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55.
7. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
8. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
9. Pasqualucci, L. et al. (2001) Nature 412, 341-6.
10. Kim, O. et al. (2004) Oncogene 23, 1838-44.
11. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
12. Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
13. Feldman, J.D. et al. (1998) J Biol Chem 273, 16535-43.
14. Li, Y.Y. et al. (2006) Cancer Res 66, 6741-7.
15. Fujii, C. et al. (2005) Int J Cancer 114, 209-18.
16. Popivanova, B.K. et al. (2007) Cancer Sci 98, 321-8.
17. Zheng, H.C. et al. (2008) J Cancer Res Clin Oncol 134, 481-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Pim-3 (D17C9) Rabbit mAb detects endogenous levels of total Pim-3 protein. It does not cross-react with other Pim family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser275 of human Pim-3.

Background

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12).Pim-3, originally named Kid-1, was identified as a protein induced by depolarization in PC-12 cells (13). Like other family members, Pim-3 can phosphorylate Bad to inhibit Bad-mediated apoptosis (14). Aberrant expression of Pim-3 has been observed in a number of types of cancer, including liver, pancreas, colon and gastric cancer (14-17).

1. Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15.
2. Selten, G. et al. (1986) Cell 46, 603-11.
3. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12.
4. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20.
5. Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
6. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55.
7. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
8. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
9. Pasqualucci, L. et al. (2001) Nature 412, 341-6.
10. Kim, O. et al. (2004) Oncogene 23, 1838-44.
11. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
12. Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
13. Feldman, J.D. et al. (1998) J Biol Chem 273, 16535-43.
14. Li, Y.Y. et al. (2006) Cancer Res 66, 6741-7.
15. Fujii, C. et al. (2005) Int J Cancer 114, 209-18.
16. Popivanova, B.K. et al. (2007) Cancer Sci 98, 321-8.
17. Zheng, H.C. et al. (2008) J Cancer Res Clin Oncol 134, 481-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody