DHX29 Antibody

• 产品编号：CST-4159S      品牌：CST       原厂货号：4159S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DHX29 Antibody检测内源性DHX29总蛋白水平。通过人工合成人源DHX29蛋白羧基末端相应序列的多肽片段，使用该片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。DHX29蛋白是一种ATP依赖的RNA解旋酶，该酶属于DEAD-box解旋酶家族(DEAH亚家族)。DHX29包含一个中心解旋酶和一个羧基末端结构域的解旋酶(1)。DHX29蛋白的功能还没有完全研究透彻，但是最近研究显示该蛋白通过mRNAs的5'非翻译区域促进翻译起始(2)。DHX29蛋白可结合40S亚单位，并且水解ATP、GTP、UTP和CTP。通过43S复合物可强烈刺激DHX29蛋白的核苷三磷酸水解作用，同时在DHX29蛋白促进48S复合物的形成过程中，上述水解过程对于DHX29蛋白活性是必须的(2)。

1. de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8.
2. Pisareva, V.P. et al. (2008) Cell 135, 1237-50.

原厂资料：

Specificity / Sensitivity

DHX29 Antibody detects endogenous levels of total DHX29 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the C-terminus of human DHX29. Antibodies were purified by protein A and peptide affinity chromatography.

Background

DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).

1. de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8.
2. Pisareva, V.P. et al. (2008) Cell 135, 1237-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.