EPAC2 (5B1) Mouse mAb

• 产品编号：CST-4156S      品牌：CST       原厂货号：4156S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EPAC2 (5B1) Mouse mAb检测内源性EPAC2总蛋白。通过重组小鼠EPAC2蛋白片段去免疫动物从而制备出单克隆抗体。EPAC1和EPAC2 (exchange proteins activated by cyclic AMP)蛋白是鸟嘌呤核苷酸交换因子(GEFs)，它能催化GDP转化成GTP、活化Rap1和Rap2小GTPases。通过EPAC活化的Rap蛋白是cAMP依赖的，并且通过蛋白酶A(protein kinase A,PKA)在某种程度上介导cAMP的信号（1）。EPAC 蛋白信号在许多细胞内进程包括迁移和粘着斑形成(2)、胞外分泌(3)、胰岛素信号(4)、轴突生长和导向(5)以及神经递质释放(6)中起着重要作用。

1. Bos, J.L. (2006) Trends Biochem Sci 31, 680-6.
2. Lyle, K.S. et al. (2008) Cell Signal 20, 1104-16.
3. Branham, M.T. et al. (2009) J Biol Chem 284, 24825-39.
4. Petersen, R.K. et al. (2008) Mol Cell Biol 28, 3804-16.
5. Murray, A.J. and Shewan, D.A. (2008) Mol Cell Neurosci 38, 578-88.
6. Ouyang, M. et al. (2008) Proc Natl Acad Sci USA 105, 11993-7.

原厂资料：

Specificity / Sensitivity

EPAC2 (5B1) Mouse mAb detects endogenous levels of total EPAC2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of mouse EPAC2.

Background

EPAC1 and EPAC2 (exchange proteins activated by cyclic AMP) are guanine nucleotide exchange factors (GEFs) that catalyze the exchange of GDP for GTP, activating Rap1 and Rap2 small GTPases. Rap activation by EPAC is cAMP-dependent and mediates cAMP signaling in part through protein kinase A (PKA) (reviewed in 1). EPAC signaling plays a significant role in a number of cellular processes including migration and focal adhesion formation (2), exocytosis (3), insulin signaling (4), axon growth and guidance (5) and neurotransmitter release (6).

1. Bos, J.L. (2006) Trends Biochem Sci 31, 680-6.
2. Lyle, K.S. et al. (2008) Cell Signal 20, 1104-16.
3. Branham, M.T. et al. (2009) J Biol Chem 284, 24825-39.
4. Petersen, R.K. et al. (2008) Mol Cell Biol 28, 3804-16.
5. Murray, A.J. and Shewan, D.A. (2008) Mol Cell Neurosci 38, 578-88.
6. Ouyang, M. et al. (2008) Proc Natl Acad Sci USA 105, 11993-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.