STIM1 (G555) Antibody

• 产品编号：CST-4149S      品牌：CST       原厂货号：4149S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

STIM1 (G555)抗体识别内源性的全部STIM1蛋白。该多克隆抗体用与人类STIM1中Gly555位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。Ca2+是许多细胞内信号转导通路中关键的第二信使。钙离子信号控制许多细胞功能，包括短期反应如收缩和分泌，以及长期调节如细胞生长和增殖（1,2）。基质相互作用分子（STIMs）作为钙传感器，可以检测细胞内Ca2+储存库中Ca2+含量的变化（3）。STIM1是保守的，有广泛的表达，作为内质网（ER）Ca2+传感器，当钙存储不足时从内质网钙库中迁移到细胞膜，激活钙释放激活的钙通道（CRAC）（4）。STIM1是一个潜在的肿瘤抑制因子；STIM1缺陷可能导致横纹肌肉瘤和横纹肌样瘤（5）。STIM1可以自身二聚化或与STIM2形成异二聚体。。STIM2与STIM1具有较高的同源性，可以作为抑制剂而抑制STIM1介导的细胞膜钙库控制的钙离子的进入（6）。然而，STIM2真正的生理功能尚需要进一步研究。

1. Berridge, M.J. et al. (2000) Nat Rev Mol Cell Biol 1, 11-21.
2. Berridge, M.J. et al. (2003) Nat Rev Mol Cell Biol 4, 517-29.
3. Zheng, L. et al. (2008) Biochem Biophys Res Commun 369, 240-6.
4. Zhang, S.L. et al. (2005) Nature 437, 902-5.
5. Manji, S.S. et al. (2000) Biochim Biophys Acta 1481, 147-55.
6. Soboloff, J. et al. (2006) Curr Biol 16, 1465-70.

原厂资料：

Specificity / Sensitivity

STIM1 (G555) Antibody detects endogenous levels of total STIM1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the region surrounding Gly555 of human STIM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ca2+ is a key second messenger in many intracellular signaling pathways. Ca2+ signals control many cellular functions ranging from short-term responses such as contraction and secretion to longer-term regulation of cell growth and proliferation (1,2). Stromal interaction molecules (STIMs) function as Ca2+ sensors that detect changes in Ca2+ content in intracellular Ca2+ stores (3). STIM1 is conserved, ubiquitously expressed, and functions as an endoplasmic reticulum (ER) Ca2+ sensor that migrates from the ER Ca2+ store to the plasma membrane where it activates calcium-release-activated calcium (CRAC) channels when the ER Ca2+ store is low (4). STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors (5). STIM1 can either homodimerize or form heterodimers with STIM2. STIM2 possesses a high sequence identity to STIM1 and can function as an inhibitor of STIM1-mediated plasma membrane store-operated Ca2+ entry (6). However, further investigation is required to elucidate the true physiological function of STIM2.

1. Berridge, M.J. et al. (2000) Nat Rev Mol Cell Biol 1, 11-21.
2. Berridge, M.J. et al. (2003) Nat Rev Mol Cell Biol 4, 517-29.
3. Zheng, L. et al. (2008) Biochem Biophys Res Commun 369, 240-6.
4. Zhang, S.L. et al. (2005) Nature 437, 902-5.
5. Manji, S.S. et al. (2000) Biochim Biophys Acta 1481, 147-55.
6. Soboloff, J. et al. (2006) Curr Biol 16, 1465-70.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.