Phospho-Numb (Ser276) Antibody

• 产品编号：CST-4140S      品牌：CST       原厂货号：4140S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Numb (Ser276) 抗体只识别Ser276磷酸化的内源性Numb蛋白.多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Numb蛋白Ser276氨基酸残基序列一致。抗体由肽段由蛋白A和亲和层析技术纯化。Numb包含一个氨基末端磷酸化酪氨酸结合（PTB）结构域和C末端对α-衔接蛋白和EH（Eps15 同源物）结构域包含蛋白的内吞结合基序，显示在细胞吞噬中的作用（1,2）有四个哺乳动物Numb剪接异构体，差异表达，并有各自独特功能（3-5）。Numb通过促进Notch泛素降解对Notch信号通路起负向调控作用（6），蛋白在细胞分裂时不对称的入子细胞，产生的两个子细胞对Notch信号和细胞命运都有不同反应（7,8）。Numb可能被G蛋白偶联受体和PKC信号调控（9）。Numb可能在不同的位点磷酸化，包括丝氨酸7，丝氨酸295和丝氨酸276。在这些位点的磷酸化调控Numb非对称膜定位和整合内吞作用（10-12）.

1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.
10. Nishimura, T. and Kaibuchi, K. (2007) Dev Cell 13, 15-28.
11. Smith, C.A. et al. (2007) EMBO J. 26, 468-480.
12. Wirtz-Peitz, F. et al. (2008) Cell 135, 161-173.

原厂资料：

Specificity / Sensitivity

Phospho-Numb (Ser276) Antibody detects endogenous levels of Numb protein only when phosphorylated on Ser276.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to Ser276 of the human Numb protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).Numb can be phosphorylated at several sites including Ser7, Ser276 and Ser295. Phosphorylation at these sites regulates asymmetric membrane localization of Numb and integrin endocytosis (10-12).

1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.
10. Nishimura, T. and Kaibuchi, K. (2007) Dev Cell 13, 15-28.
11. Smith, C.A. et al. (2007) EMBO J. 26, 468-480.
12. Wirtz-Peitz, F. et al. (2008) Cell 135, 161-173.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody