Cyclin E2 Antibody

• 产品编号：CST-4132P      品牌：Cell Signaling Technology       原厂货号：4132P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cyclin E2 Antibody 能够检测内源性cyclin E2总蛋白。该单克隆抗体是由合成的人源的针对cyclin E2蛋白羧基末端肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。Cyclin E1和Cyclin E2协同并激活CDK2(1)。DNA损伤时，上调/激活CDK抑制基因p21 Waf1/Cip1 and p27 Kip1能够阻滞cyclin E/CDK2的激活，从而导致G1/S期停滞。当条件适宜于细胞周期连续进行时，cyclin D/CDK4/6使Rb磷酸化从而能够降低p21 Waf1/Cip1 和 p27 Kip1的活性，使得cyclin E/CDK2随后激活(1,2)。Cyclin E/CDK2进一步磷酸化Rb使细胞周期进入S期，在此期内cyclin E/CDK2被认为可以磷酸化并激活涉及DNA合成过程的多种蛋白(2,3)。周期蛋白E的功能转化主要受磷酸化控制，这种磷酸化能够导致SCFFbw7介导的泛素化和蛋白酶体依赖的降解(4,5)。Cyclin E1在体内可以在包括苏氨酸62位，丝氨酸88位，丝氨酸72位，苏氨酸380位和丝氨酸384位的多位点磷酸化，这个磷酸化过程至少可以被两个激酶-CDK2和GSK-3调控

1. Lauper, N. et al. (1998) Oncogene 17, 2637-2643.
2. Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761.
3. Ewen, M.E. (2000) Genes Dev. 14, 2265-2270.
4. Won, K.A. and Reed, S.I. (1996) EMBO J. 15, 4182-4193.
5. Koepp, D.M. et al. (2001) Science 294, 173-177.
6. Welcker, M. et al. (2003) Mol. Cell 12, 381-392.
7. Ye, X. et al. (2004) J. Biol. Chem. 279, 50110-50119.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

Cyclin E2 Antibody detects endogenous levels of total cyclin E2 protein. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human cyclin E2. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, 

1. Lauper, N. et al. (1998) Oncogene 17, 2637-2643.
2. Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761.
3. Ewen, M.E. (2000) Genes Dev. 14, 2265-2270.
4. Won, K.A. and Reed, S.I. (1996) EMBO J. 15, 4182-4193.
5. Koepp, D.M. et al. (2001) Science 294, 173-177.
6. Welcker, M. et al. (2003) Mol. Cell 12, 381-392.
7. Ye, X. et al. (2004) J. Biol. Chem. 279, 50110-50119.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.