Phospho-Cyclin B1 (Ser147) Antibody

• 产品编号：CST-4131S      品牌：CST       原厂货号：4131S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Cyclin B1 (Ser147) Antibody能够检测内源性的丝氨酸（147位点）磷酸化的cyclin B1蛋白。该单克隆抗体是由合成的人源的针对cyclin B1蛋白丝氨酸(147位)磷酸化肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。cdc2蛋白激酶的激活参与调节所有的真核细胞进入有丝分裂。cdc2的激活受多个步骤的调控，包括周期蛋白B的结合、cdc2苏氨酸161位的磷酸化和苏氨酸14位和15位的去磷酸化(1-5)。CDK抑制剂和CDK相关的细胞周期蛋白的水平可通过磷酸化、泛素化和降解来调节，允许了细胞周期事件的化学计量调节(6)。四个周期蛋白B1磷酸化位点(丝氨酸126位，128位，133位和147位)位于胞质滞留信号(CRS)结构域，并可以调节G2/S检验点周期蛋白B1的核迁移(8-10)。周期蛋白B1的磷酸化参与了cdc25C依赖的cdc2 酪氨酸15位的去磷酸化和随后的cdc2/周期蛋白B1 激活(11)。尽管cdc2/周期蛋白B1本身能够磷酸化丝氨酸126位和128位(8)，polo样蛋白激酶优先磷酸化丝氨酸133位，也可能磷酸化丝氨酸147位(11-13)。

1. Lorca, T. et al. (1992) EMBO J 11, 2381-90.
2. Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9.
3. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
5. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
6. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
7. Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7.
8. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
9. Santos, S.D. et al. (2012) Cell 149, 1500-13.
10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
12. Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63.
13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

原厂资料：

omology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

Phospho-Cyclin B1 (Ser147) Antibody detects endogenous levels of cyclin B1 only when phosphorylated at serine 147. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser147 of human cyclin B1. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13). 

1. Lorca, T. et al. (1992) EMBO J 11, 2381-90.
2. Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9.
3. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
5. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
6. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
7. Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7.
8. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
9. Santos, S.D. et al. (2012) Cell 149, 1500-13.
10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
12. Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63.
13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.