Ape1 Antibody

• 产品编号：CST-4128S      品牌：CST       原厂货号：4128S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Ape1抗体能够检测内源性Ape1总蛋白水平。该多克隆抗体由合成的人源的针对Ape1蛋白近丙氨酸(230位)的肽段免疫动物，利用多肽亲和层析的方法纯化获得。 Ape1(脱嘌呤/脱嘧啶核酸内切酶1)，又称Ref1(氧化还原效应因子1)，是一种多功能蛋白，具有多种生物学活性，主要包括在DNA修复和细胞氧化应激反应中的作用。Ape1启动脱碱基位点的修复并在碱基切除修复(BER)路径中发挥重要作用(1)。Ape1的修复活性通过与BER中另外一个关键蛋白XRCC1 (2)相互作用被激活。作为一个重要的氧化还原因子，Ape1不仅可以维持转录因子在一个活跃还原的状态，也可以独立于氧化还原的形式，作为转录辅助因子控制细胞命运，如细胞凋亡、增殖和分化(3)。Ape1表达的升高和多种类型的肿瘤相关，如子宫颈癌、卵巢癌、前列腺癌、横纹肌肉瘤和生殖细胞肿瘤(4)。Ape1可以促进DNA结合肿瘤发生过程中几个重要的转录因子如Fos, Jun, NF-κB, PAX, HIF-1, HLF 和 p53 (4)。Ape1基因的突变也和肌萎缩侧索硬化症有关(5,6)。

1. Demple, B. and Sung, J.S. (2005) DNA Repair (Amst) 4, 1442-9.
2. Vidal, A.E. et al. (2001) EMBO J 20, 6530-9.
3. Tell, G. et al. Antioxid Redox Signal 7, 367-84.
4. Evans, A.R. et al. (2000) Mutat Res 461, 83-108.
5. Olkowski, Z.L. (1998) Neuroreport 9, 239-42.
6. Hayward, C. et al. (1999) Neurology 52, 1899-901.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Ape1 Antibody detects endogenous levels of total Ape1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ala230 of human Ape1. Antibodies are purified by peptide affinity chromatography. 

Background

Ape1 (Apurinic/Apyrimidic eEndonuclease 1), also known as Ref1 (Redox effector factor 1), is a multifunctional protein with several biological activities. These include roles in DNA repair and in the cellular response to oxidative stress. Ape1 initiates the repair of abasic sites and is essential for the base excision repair (BER) pathway (1). Repair activities of Ape1 are stimulated by interaction with XRCC1 (2), another essential protein in BER. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state but can also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation (3). Increased expression of Ape1 is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors (4). Ape1 has been shown to stimulate DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-κB, PAX, HIF-1, HLF and p53 (4). Mutation of the Ape1 gene has also been associated with amyotrophic lateral sclerosis (ALS) (5,6).

1. Demple, B. and Sung, J.S. (2005) DNA Repair (Amst) 4, 1442-9.
2. Vidal, A.E. et al. (2001) EMBO J 20, 6530-9.
3. Tell, G. et al. Antioxid Redox Signal 7, 367-84.
4. Evans, A.R. et al. (2000) Mutat Res 461, 83-108.
5. Olkowski, Z.L. (1998) Neuroreport 9, 239-42.
6. Hayward, C. et al. (1999) Neurology 52, 1899-901.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.