Phospho-TIF1β (Ser824) Antibody

• 产品编号：CST-4127S      品牌：CST       原厂货号：4127S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-TIF1β (Ser824) Antibody能够检测内源性丝氨酸（824位）磷酸化的TIF1β 蛋白。该多克隆抗体是由合成的人源的针对TIF1β蛋白丝氨酸（824位）的磷酸化肽段免疫动物，采用多肽亲和层析技术纯化生产的。TIF1β是转录中间因子TIF1家族的一员，属于在发育和分化中起着关键作用的转录调节子。该家族成员的主要特征在于两个保守的基序一个氨基末端的RING-B盒卷曲螺旋基序和一个羧基末端的PHD手指和溴区单元(1,2)。TIF1β是含有锌指蛋白的KRAB(Kruppel 相关盒)结构域的辅助抑制物。含有锌指基序的KRAB结构域是转录因子的一大组，为脊椎动物所特有，其表达模式在不同物种间不同，被认为能够调节控制形态的基因转录程序(3,4)。TIF1β已经被证实为早期胚胎发育和精子发生所必需(6,5)。其功能是激活或抑制转录以响应环境或发育信号，主要通过染色质重构和组蛋白修饰。TIF1β的招募以及和异染色质蛋白(HP1)的联合是转录抑制必不可少的，而且为F9胚胎肿瘤细胞的分化所必需(6,7)。TIF1β也在DNA损伤反应中起作用。TIF1β的第842位丝氨酸发生ATM依赖方式的磷酸化应答基因毒性应激且为染色体松弛所必需，转而为DNA损伤反应所必需(8)。

1. Le Douarin, B. et al. (1995) EMBO J. 14, 2020-2033.
2. Le Douarin, B. et al. (1996) EMBO J. 15, 6701-6715.
3. Friedman, J.R. et al. (1996) Genes Dev. 10, 2067-2078.
4. Krebs, C.J. et al. (2005) Genomics 85, 752-761.
5. Weber, P. et al. (2002) Development 129, 2329-2337.
6. Cammas, F. et al. (2004) Genes Dev. 18, 2147-2160.
7. Cammas, F. et al. (2007) Differentiation 75, 627-37.
8. Ziv, Y. et al. (2006) Nat. Cell Biol. 8, 870-876.

原厂资料：

Specificity / Sensitivity

Phospho-TIF1β (Ser824) Antibody detects endogenous levels of TIF1β protein only when phosphorylated on Ser824.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids surrounding Ser824 of human TIF1β. Antibodies are purified by peptide affinity chromatography.

Background

TIF1β is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1β is a corepressor for KRAB (Kruppel associated box) domain containing zinc finger proteins. The KRAB domain containing zinc finger proteins are a large group of transcription factors that are vertebrate-specific, varied in their expression patterns between species, and thought to regulate gene transcription programs that control speciation (3,4). 
 TIF1β has been shown to be essential for early embryonic development and spermatogenesis (6,5). It functions to either activate or repress transcription in response to environmental or developmental signals by chromatin remodeling and histone modification. The recruitment and association of TIF1β with heterochromatin protein (HP1) is essential for transcriptional repression, and for progression through differentiation of F9 embryonic carcinoma cells (6,7). TIF1β also plays a role in the DNA damage response. Phosphorylation of TIF1β on Ser842 occurs in an ATM-dependent manner in response to genotoxic stress and is thought to be essential for chromatin relaxation, which is in turn required for the DNA damage response (8).

1. Le Douarin, B. et al. (1995) EMBO J. 14, 2020-2033.
2. Le Douarin, B. et al. (1996) EMBO J. 15, 6701-6715.
3. Friedman, J.R. et al. (1996) Genes Dev. 10, 2067-2078.
4. Krebs, C.J. et al. (2005) Genomics 85, 752-761.
5. Weber, P. et al. (2002) Development 129, 2329-2337.
6. Cammas, F. et al. (2004) Genes Dev. 18, 2147-2160.
7. Cammas, F. et al. (2007) Differentiation 75, 627-37.
8. Ziv, Y. et al. (2006) Nat. Cell Biol. 8, 870-876.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody