Microcephalin-1/BRIT1 (D38G5) Rabbit mAb

• 产品编号：CST-4120S      品牌：CST       原厂货号：4120S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Microcephalin-1/BRIT1 (D38G5) Rabbit mAb能够检测内源性Microcephalin-1/BRIT1总蛋白。 该单克隆抗体是由合成的人源的针对Microcephalin-1/BRIT1蛋白中间序列肽段免疫动物生产的。 微脑磷脂-1 (MCPH1)/BRIT1是一种早期DNA损伤反应蛋白，以其在人类原发性小颅疾病中的突变状态而得名。BRIT1形成的损伤-诱导核灶参与DNA损伤和细胞周期检验点并调节有丝分裂。BRIT1在DNA损伤反应中必不可少，其缺失可以导致基因组不稳定性。作为一个潜在的肿瘤抑制基因，BRIT1在人类癌症中的表达是减少的(回顾1-2, 3)。BRIT1和其它的DNA修复蛋白(53BP1, MDC1, NBS1, ATM, RPA, ATR)共定位而且对于它们的激活时必须的(2)。BRIT1可能是通过响应DAN损伤的染色质重构来调节DNA修复，允许修复蛋白进入DNA(4)。

1. Lin, S.Y. et al. (2005) Proc Natl Acad Sci U S A 102, 15105-9.
2. Rai, R. et al. (2006) Cancer Cell 10, 145-57.
3. Chaplet, M. et al. (2006) Cell Cycle 5, 2579-83.
4. Peng, G. et al. (2009) Nat Cell Biol 11, 865-72.

原厂资料：

Specificity / Sensitivity

Microcephalin-1/BRIT1 (D38G5) Rabbit mAb detects endogenous levels of total Microcephalin-1/BRIT1 protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human Microcephalin-1/BRIT1. 

Background

Microcephalin-1 (MCPH1)/BRIT1 is an early DNA damage response protein named for its mutated state in the human disease primary microcephaly. BRIT1 forms damage-induced nuclear foci, is involved in DNA damage and cell cycle checkpoints as well as regulation of mitosis. BRIT1 function is necessary for DNA damage responses, and the absence of BRIT1 function leads to genome instability. A potential tumor suppressor, BRIT1 expression is reduced in human carcinomas (1-2, reviewed in 3). 
 BRIT1 colocalizes with other DNA repair proteins (53BP1, MDC1, NBS1, ATM, RPA, and ATR) and is required for their activation (2). BRIT1 likely regulates DNA repair through chromatin remodeling in response to DNA damage, allowing access of repair proteins to DNA (4). 

1. Lin, S.Y. et al. (2005) Proc Natl Acad Sci U S A 102, 15105-9.
2. Rai, R. et al. (2006) Cancer Cell 10, 145-57.
3. Chaplet, M. et al. (2006) Cell Cycle 5, 2579-83.
4. Peng, G. et al. (2009) Nat Cell Biol 11, 865-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.