Cyclin B1 (V152) Mouse mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-4118S      品牌：CST       原厂货号：4118S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Cyclin B1 (V152) Mouse mAb (Alexa Fluor® 647 Conjugate) 能够检测内源性不依赖于磷酸化的细胞周期蛋白B1。该单克隆抗体是由针对仓鼠周期蛋白B1的一段肽段免疫动物而生产的。在最适宜的条件下，当F/P比例为2-5时，将该抗体与Alexa Fluor® 647偶联。Alexa Fluor® 647 染料由红光最大限度激发（如633nm HeNe 激光）。与 Alexa Fluor® 647 偶联的抗体可以产生明亮远红荧光激发光，其峰值在665nm处。该抗体偶联于Alexa Fluor®647 荧光染料，并经过内部测试，直接进行人类细胞的流式细胞术分析。货号为#4135，未偶联荧光染料的Cyclin B1抗体可以识别人、小鼠和仓鼠周期蛋白B1。CST期望 Cyclin B1 Mouse mAb (Alexa Fluor®647 conjugate) 同样可以识别以上物种的周期蛋白B1。 cdc2蛋白激酶的激活参与调节所有的真核细胞进入有丝分裂。cdc2的激活受多个步骤的调控，包括周期蛋白B的结合、cdc2苏氨酸161位的磷酸化和苏氨酸14位和15位的去磷酸化(1-5)。CDK抑制剂和CDK相关的细胞周期蛋白的水平可通过磷酸化、泛素化和降解来调节，允许了细胞周期事件的化学计量调节(6)。四个周期蛋白B1磷酸化位点(丝氨酸126位，128位，133位和147位)位于胞质滞留信号(CRS)结构域，并可以调节G2/S检验点周期蛋白B1的核迁移(8-10)。周期蛋白B1的磷酸化参与了cdc25C依赖的cdc2 酪氨酸15位的去磷酸化和随后的cdc2/周期蛋白B1 激活(11)。尽管cdc2/周期蛋白B1本身能够磷酸化丝氨酸126位和128位(8)，polo样蛋白激酶优先磷酸化丝氨酸133位，也可能磷酸化丝氨酸147位(11-13)。

1. Lorca, T. et al. (1992) EMBO J 11, 2381-90.
2. Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9.
3. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
5. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
6. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
7. Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7.
8. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
9. Santos, S.D. et al. (2012) Cell 149, 1500-13.
10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
12. Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63.
13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

原厂资料：

omology

Species predicted to react based on 100% sequence homology: Hamster

Specificity / Sensitivity

Cyclin B1 (V152) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of cyclin B1 independent of phosphorylation. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a peptide corresponding to a sequence from hamster cyclin B1. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-5. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Description

Cell Signaling Technology Antibody conjugated to Alexa Fluor®647 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human cells. The unconjugated antibody #4135 reacts with, among others, human, mouse and hamster Cyclin B1. CST expects that Cyclin B1 Mouse mAb (Alexa Fluor®647 conjugate) will also recognize Cyclin B1 in these species. 

Background

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13).

1. Lorca, T. et al. (1992) EMBO J 11, 2381-90.
2. Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9.
3. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
5. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
6. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
7. Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7.
8. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
9. Santos, S.D. et al. (2012) Cell 149, 1500-13.
10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
12. Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63.
13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze.