CDCP1 Antibody

• 产品编号：CST-4115S      品牌：CST       原厂货号：4115S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDCP1抗体检测内源性CDCP1蛋白水平，140kDa条带是全长，糖基化的CDCP1；70kDa条带是CDCP1的剪切产物。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于人源CDCP1蛋白C末端附近残基相对应。抗体由肽段亲和层析技术纯化。CDCP1（补体-Uegt-Bmp-1 结构域包含蛋白，SIMA135）可能是一个干细胞标志物，并在某些癌症细胞中，典型和非典型（癌症）结肠中高度表达(1)。CDCP1可能受到表观遗传调控,随启动子CqG序列甲基化导致CDCP1表达减少（2）。相应的CDCP1基因编码依他糖蛋白，此糖蛋白以复合物多结构域跨膜抗原行使作用。三个CUB结构域在胞外区域，很可能涉及细胞粘附或胞外基质相互作用（1,3）。Src家族激酶可能在CDCP1胞质结构域的五个酪氨酸残基磷酸化，以提供潜在绑定位点给SH2-结构域蛋白（3）。CDCP1可能是肝脏干细胞标志物（4,5）。

1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

原厂资料：

Specificity / Sensitivity

CDCP1 Antibody detects endogenous levels of total CDCP1 protein. The 140kDa band is full length, glycosylated CDCP1, the 70kDa band is a cleavage product of CDCP1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy-terminus of human CDCP1. Antibodies are purified by peptide affinity chromatography.

Background

CDCP1 (CUB domain containing protein, SIMA135) is a putative stem cell marker that is highly expressed in some human cancer cells and in both, typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. Three CUB domains in its extracellular region are possibly involved in cell adhesion or extracellular matrix interaction (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2- domain containing proteins (3). CDCP1 is a putative marker for hematopoietic stem cells (4,5).

1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.