Ku70 (V540) Antibody

• 产品编号：CST-4104S      品牌：CST       原厂货号：4104S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Ku70 (V540) Antibody能够检测内源性Ku70总蛋白。该多克隆抗体是由合成的人源的Ku70蛋白羧基末端的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。Ku是由两个亚基(Ku70 and Ku80)组成的异源二聚体蛋白，最初在几种自体免疫疾病包括硬皮病、多发性肌炎和系统性红斑狼疮中作为自体抗原被鉴定出来(1)。Ku是一种高丰度、广泛表达的核蛋白，它能够结合并稳定端粒或双链DNA断裂处的DNA末端(2-5)。Ku70/Ku80异源二聚体具有ATP-依赖的DNA解旋酶活性，并能够作为DNA-依赖蛋白激酶(DNA-PK)的DNA-结合调节成分发挥作用(6-8)。DNA末端DNA-PK复合体的装配需要非同源末端连接(NHEJ)，一种涉及双链DNA断裂修复和V(D)J重组的机制(8)。DNA-PK能够磷酸化许多蛋白，包括p53，血清反应因子，c-Jun，c-Fos，c-Myc，Oct-1，Sp-1 和RNA 聚合酶 II (1,8)。Ku70/Ku80和DNA-PK的联合活性暗示了Ku在多种细胞功能中的作用，包括细胞周期调控、DNA复制和修复、端粒维持、重组和转录激活。

1. Tuteja, R. and Tuteja, N. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 1-33.
2. Blier, P.R. et al. (1993) J. Biol. Chem. 268, 7594-7601.
3. Jin, S. and Weaver, D.T. (1997) EMBO J. 16, 6874-6885.
4. Boulton, S.J. and Jackson, S.P. (1998) EMBO J. 17, 1819-1828.
5. Gravel, S. et al. (1998) Science 280, 741-744.
6. Cao, Q.P. et al. (1994) Biochemistry 33, 8548-8557.
7. Lees-Miller, S.P. et al. (1990) Mol. Cell Biol. 10, 6472-6481.
8. Collis, S.J. et al. (2005) Oncogene 24, 949-961.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster

Specificity / Sensitivity

Ku70 (V540) Antibody detects endogenous levels of total Ku70 protein. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human Ku70 protein. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks (2-5). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). DNA-PK has been shown to phosphorylate many proteins, including p53, serum response factor, c-Jun, c-Fos, c-Myc, Oct-1, Sp-1 and RNA polymerase II (1,8). The combined activities of Ku70/Ku80 and DNA-PK implicate Ku in many cellular functions, including cell cycle regulation, DNA replication and repair, telomere maintenance, recombination and transcriptional activation.

1. Tuteja, R. and Tuteja, N. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 1-33.
2. Blier, P.R. et al. (1993) J. Biol. Chem. 268, 7594-7601.
3. Jin, S. and Weaver, D.T. (1997) EMBO J. 16, 6874-6885.
4. Boulton, S.J. and Jackson, S.P. (1998) EMBO J. 17, 1819-1828.
5. Gravel, S. et al. (1998) Science 280, 741-744.
6. Cao, Q.P. et al. (1994) Biochemistry 33, 8548-8557.
7. Lees-Miller, S.P. et al. (1990) Mol. Cell Biol. 10, 6472-6481.
8. Collis, S.J. et al. (2005) Oncogene 24, 949-961.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody.