DRAQ5?

• 产品编号：CST-4084L      品牌：CST       原厂货号：4084L
• 产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 细胞核酸染料
• 应用分类：

描述：

请参照CST公司建议的IF和Flow步骤，对于两种方法，请使用以下检测： 流式细胞术：离心细胞，用按照1:5000 (1 µM)将 DRAQ5 ® 加到0.5ml的孵育缓冲液中。避光室温孵育5分钟，然后采用流式细胞术分析细胞。 免疫荧光：用PBS清洗细胞2次，每次5分钟。按照1:1000（5uM）用PBS稀释e DRAQ5 ®，避光室温孵育5分钟，用PBS清洗细胞一次，盖上带有褪色试剂涂层盖玻片，采用适当的激发光波长尽快检测细胞。本产品是一种能透过细胞膜的远红DNA荧光染料，可以应用在肝脏中或用来固定细胞。这种染料可以与GFP或FITC标记结合。 DRAQ5 ® 在流式细胞术和荧光显微镜中检测细胞内的DNA（1-3）。

1. Smith, P.J. et al. (2000) Cytometry 40, 280-91.
2. Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
3. Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.

原厂资料：

Directions for Use

Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection: 
 Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer. 
 Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

Background

DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).

1. Smith, P.J. et al. (2000) Cytometry 40, 280-91.
2. Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
3. Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.

注意事项：

Storage: Store at 4ºC protected from light.
Directions for Use: Please follow CST’s recommended IF
and Flow p