DAPI

产品编号：CST-4083S 品牌：CST 原厂货号：4083S
产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 细胞核酸染料
应用分类：

包装：

运保温度:

4°C

到货周期：

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描述：

本产品溶于去离子水中的最大浓度为50mg/ml，溶于二甲基甲酰胺中为澄明黄色液体。需要解热和（或）超生。对于标准荧光为基础的分析方法，DAPIstock 溶液需溶于磷酸盐缓冲液中或封片液中，终浓度为1到0.01ug/ml。DAPI是一种蓝色的荧光染料，靶向检测在DNA凹槽中的双链AT团（1）。染料上的一个分子与dsDNA上的每3个碱基对结合，达到大约20倍的荧光强度（2）。DAPI-DNA的荧光水平与DNA的含量相关（3）

原厂资料：

Directions for Use

This product is soluble up to 20 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, DAPI stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 to 0.01 μg/mL.

Background

4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).

注意事项：

Storage: Store lyophilized or in solution at 4°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 2-3 weeks to prevent
loss of potency. Aliquot to avoid multiple freeze/thaw cycles.