Hoechst 33342

• 产品编号：CST-4082S      品牌：CST       原厂货号：4082S
• 产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 细胞核酸染料
• 应用分类：

描述：

本产品溶于去离子水中的最大浓度为50mg/ml，溶于二甲基甲酰胺 中为澄明黄色液体。需要解热和（或）超生。对于标准荧光为基础的分析方法，Hoechst 33342 stock 溶液需溶于磷酸盐缓冲液中或封片液中，终浓度为1ug/ml。Hoechst 33342 and Hoechst 33258是一种荧光DNA染料，能够特异性地与双链富含AT的区域的凹槽结合。这些染料可以用于活的和已固定的细胞（1），正在复制或分裂的染色体不能使用Hoechst 33342染料。

原厂资料：

Directions for Use

This product is soluble up to 50 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml.

Background

Hoechst 33342 and Hoechst 33258 are specific fluorescent DNA stains that bind within the minor groove of double-stranded AT-rich regions. These stains can be used on both live and fixed cells (1). Replicating or dividing chromosomes will not stain with Hoechst 33342 DNA dye.

注意事项：

Storage: The lyophilized powder is stable for 1 year if kept
below 0ºC with desiccant. Aqueous solutions are stable for at
least 1 month if kept in the dark between 2-8ºC.