Pan-Cadherin Antibody

• 产品编号：CST-4068S      品牌：CST       原厂货号：4068S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Pan-Cadherin抗体检测内源性E-, P-, N-, R-, VE-和 K-Cadherins的水平 。多抗由两个合成肽段免疫动物产生，这两个肽段对应人N-Cadherin的两个保守区域，抗体由蛋白A和肽段亲和层析技术纯化得到。Cadherins是跨膜糖蛋白超家族，含在胞外结构域约100个残基的Cadherin重复单元，Cadherins 调节钙依赖的细胞与细胞间的粘附，并在正常组织发育中起重要作用（1）。经典Cadherins亚家族包括N-, P-, R-, B- 和 E-cadherins，以及在细胞粘着连接中发现的近十个其他成员，粘着连接是在急性上皮细胞顶端表面附近的一种细胞结构。经典cadherins的细胞质结构域与β-catenin, γ-catenin(也称plakoglobin)，p120 catenin相互作用. β-catenin和γ-catenin与α-catenin有关，α-catenin联系cadherin-catenin复合物到肌动蛋白细胞骨架上（1,2）。β- and γ-catenin在连接复合物中起机构性作用，p120调控cadherin粘附活性和运输（1-4）。E-cadherin被认为是一种活跃的上皮癌症侵袭和增长的抑制因子（1-3）。近来研究显示癌细胞上调N-cadherin和E-cadherin减少。这种cadherin的表达变化被称作cadherin开关。N-cadherin与FGF受体协作，导致MMP9的过表达和细胞侵袭（3）。内皮细胞，VE-cadherin信号通路表达，和定位与血管通透性和肿瘤血管生成正相关（5,6）。P-cadherin的表达，通常在上皮细胞中存在，也在卵巢和其他人类癌症中变化（7,8）。

1. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
2. Christofori, G. (2003) EMBO J 22, 2318-23.
3. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34.
5. Rabascio, C. et al. (2004) Cancer Res 64, 4373-7.
6. Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7.
7. Patel, I.S. et al. (2003) Int J Cancer 106, 172-7.
8. Sanders, D.S. et al. (2000) J Pathol 190, 526-30.

原厂资料：

Specificity / Sensitivity

Pan-Cadherin Antibody detects endogenous levels of E-, P-, N-, R-, VE-, and K-Cadherins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to two conserved regions of human N-Cadherin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

1. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
2. Christofori, G. (2003) EMBO J 22, 2318-23.
3. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34.
5. Rabascio, C. et al. (2004) Cancer Res 64, 4373-7.
6. Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7.
7. Patel, I.S. et al. (2003) Int J Cancer 106, 172-7.
8. Sanders, D.S. et al. (2000) J Pathol 190, 526-30.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.