WWOX Antibody

• 产品编号：CST-4045S      品牌：CST       原厂货号：4045S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

WWOX Antibody能够检测内源WWOX总蛋白。该多克隆抗体是由合成的WWOX蛋白Thr103处周围残基相应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。WWOX（含WW结构域的氧化还原酶）基因编码一个含有两个WW结构域和一个短链脱氢酶结构域的蛋白，它来源于一个高不稳定的基因组区域16q23，FRA16D（1,2）。其小鼠同源基因称为WOX1，研究发现其能增强TNF-α介导的细胞凋亡（3）。WWOX基因在许多类型的癌症中的表达被缺失或易位作用阻断，这揭示了一个肿瘤抑制功能（4-7）。与此相反， WWOX在转移性癌症中，包括乳腺癌和前列腺癌中，已经显示出了高水平的表达量（8-10）。应激刺激可诱发第一个WW结构域（Tyr33）的酪氨酸磷酸化，其次是核易位及结合，并稳定p53肿瘤抑制蛋白（11）。WWOX和p53能够以协同方式诱导细胞凋亡。WWOX酪氨酸磷酸化和核易位参与了癌症发展过程中的转移（10）。

1. Bednarek, A.K. et al. (2000) Cancer Res. 60, 2140-2145.
2. Ried, K. et al. (2000) Hum. Mol. Genet. 9, 1651-1663.
3. Chang, N.S. et al. (2001) J. Biol. Chem. 276, 3361-3370.
4. Ramos, D. and Aldaz, C.M. (2006) Adv. Exp. Med. Biol. 587, 149-159.
5. Paige, A.J. et al. (2001) Proc. Nat.l Acad. Sci. USA 98, 11417-11422.
6. Bednarek, A.K. et al. (2001) Cancer Res. 61, 8068-8073.
7. Aqeilan, R.I. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 3949-3954.
8. Driouch, K. et al. (2002) Oncogene 21, 1832-1840.
9. Watanabe, A. et al. (2003) Cancer Res. 63, 8629-8633.
10. Chang, N.S. et al. (2005) Oncogene 24, 714-723.
11. Chang, N.S. et al. (2005) J. Biol. Chem. 280, 43100-43108.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

WWOX Antibody detects endogenous levels of total WWOX protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr103 of WWOX. Antibodies were purified by peptide affinity chromatography.

Background

The WWOX (WW domain-containing oxidoreductase) gene encodes a protein with two WW domains followed by a short-chain dehydrogenase domain that was identified from a genomic region 16q23 of high instability, FRA16D (1,2). The mouse homolog, termed Wox1, was found to enhance TNFα-mediated apoptosis (3). The WWOX gene is disrupted in a many cancer types by deletions or translocation which has revealed a tumor suppressor function (4-7). In contrast, high levels of WWOX have been shown in shown in premetastic cancers, including breast and prostate (8-10). Stress stimuli can induce tyrosine phosphorylation within the first WW domain (Tyr33), followed by nuclear translocation and binding to and stabilizing the p53 tumor suppressor protein (11). WWOX and p53 can induce apoptosis in a synergistic manner. Tyrosine phosphorylation and nuclear translocation of WWOX has been implicated in the progression of cancers to metastatic states (10).

1. Bednarek, A.K. et al. (2000) Cancer Res. 60, 2140-2145.
2. Ried, K. et al. (2000) Hum. Mol. Genet. 9, 1651-1663.
3. Chang, N.S. et al. (2001) J. Biol. Chem. 276, 3361-3370.
4. Ramos, D. and Aldaz, C.M. (2006) Adv. Exp. Med. Biol. 587, 149-159.
5. Paige, A.J. et al. (2001) Proc. Nat.l Acad. Sci. USA 98, 11417-11422.
6. Bednarek, A.K. et al. (2001) Cancer Res. 61, 8068-8073.
7. Aqeilan, R.I. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 3949-3954.
8. Driouch, K. et al. (2002) Oncogene 21, 1832-1840.
9. Watanabe, A. et al. (2003) Cancer Res. 63, 8629-8633.
10. Chang, N.S. et al. (2005) Oncogene 24, 714-723.
11. Chang, N.S. et al. (2005) J. Biol. Chem. 280, 43100-43108.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.