TCL1 Antibody

• 产品编号：CST-4042S      品牌：CST       原厂货号：4042S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TCL1 抗体检测内源性TCL1 蛋白总量。该多克隆抗体是由人源的TCL1蛋白氨基末端的合成肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。TCL1 (T cell leukemia 1), MTCP1和CL1b 都属于TCL1原癌基因家族，它们编码的蛋白参与了胚胎发育、T细胞癌变、前淋巴细胞癌变和B细胞癌变等过程中的Akt 激活（1-3）。TCL1 中的Akt 相关基团能够结合Akt基因中的PH基团。 TCL-Akt低聚物复合体的形成是TCL1 共活化的前提，能够导致Akt 的磷酸化和激活。更主要的是，功能分析揭示TCL1 和Akt 的相互作用能够促进Akt 向细胞核的转运（4-6）。TCL1的晶体结构支持了以上发现，并暗示TCL1 可能参与了分子转运（7）。

1. Narducci, M.G. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11712-11717.
2. Pekarsky, P. et al. (2001) Oncogene 20, 5638-5643.
3. Hoyer, K.K. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 14392-14397.
4. Laine, J. et al. (2000) Mol. Cell 6, 395-407.
5. Laine, J. et al. (2002) J. Biol. Chem. 277, 3743-3751.
6. KŸnstle, G. et al. (2002) Mol. Cell. Biol. 22, 1513-1525.
7. Petock, J.M. et al. (2002) Scientific World Journal 2, 1876-1884.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

TCL1 Antibody detects endogenous levels of total TCL1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human TCL1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TCL1 (T cell leukemia 1), MTCP1 and TCL1b belong to the TCL1 proto-oncogene family, and their products are involved in Akt activation during embryonic development, T cell leukemias, prolymphocytic leukemias and B cell lymphomas (1-3). The Akt association domain of TCL1 binds with the PH domain of Akt. The formation of an oligomeric TCL-Akt complex is required for TCL1 coactivator function and results in phosphorylation and activation of Akt . Furthermore, functional analysis indicates that the interaction between TCL1 and Akt promotes translocation of Akt to the nucleus (4-6). These findings are supported by the crystal structure of TCL1, which suggests that TCL1 may participate in molecular transport (7).

1. Narducci, M.G. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11712-11717.
2. Pekarsky, P. et al. (2001) Oncogene 20, 5638-5643.
3. Hoyer, K.K. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 14392-14397.
4. Laine, J. et al. (2000) Mol. Cell 6, 395-407.
5. Laine, J. et al. (2002) J. Biol. Chem. 277, 3743-3751.
6. KŸnstle, G. et al. (2002) Mol. Cell. Biol. 22, 1513-1525.
7. Petock, J.M. et al. (2002) Scientific World Journal 2, 1876-1884.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.