KLF4 Antibody

• 产品编号：CST-4038S      品牌：CST       原厂货号：4038S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

KLF4抗体识别内源性的总KLF4蛋白。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源KLF4蛋白氨基末端的氨基酸残基序列一致。抗体由肽段由蛋白A和亲和层析技术纯化.KLF4是erythroid Kruppel-like factor (EKLF)多基因家族一员，在分化的表皮层中高表达（1,2）。KLF4在表皮细胞分化过程中发挥重要功能，对于正常胃稳态是必须的（2,3）。KLF4既可以作为转录抑制子也可以作为激活子，这取决于它的靶基因（4）。研究提示该蛋白的即可作为抑癌也可以作为促癌基因，这取决于肿瘤的类型，它在人头部和脖子的扁平上皮癌中表达上调，在结肠癌中低表达（5,6）。体细胞的体外重编程为胚胎样细胞是通过四个逆转录因子：Oct-3/4, Sox2, c-Myc, 和 KLF4实现的 (7)。诱导的多潜能干细胞（iPS）是研究热点，因为他们表现了多潜能干细胞的重要特征和生长性能(8,9).

1. Yet, S.F. et al. (1998) J Biol Chem 273, 1026-31.
2. Segre, J.A. et al. (1999) Nat Genet 22, 356-60.
3. Katz, J.P. et al. (2005) Gastroenterology 128, 935-45.
4. Evans, P.M. and Liu, C. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 554-64.
5. Foster, K.W. et al. (2005) Oncogene 24, 1491-500.
6. Rowland, B.D. and Peeper, D.S. (2006) Nat Rev Cancer 6, 11-23.
7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
8. Meissner, A. et al. (2007) Nat Biotechnol 25, 1177-81.
9. Park, I.H. et al. (2008) Nature 451, 141-6.

原厂资料：

Specificity / Sensitivity

KLF4 Antibody detects endogenous levels of total KLF4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to amino acid sequence at the amino terminus of human KLF4. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

KLF4 is a member of the erythroid Kruppel-like factor (EKLF) multigene family that is highly expressed in the differentiating layers of the epidermis (1, 2). KLF4 plays a critical role in the differentiation of epithelial cells and is essential for normal gastric homeostasis (2,3). Depending on the target gene, KLF4 can function as both a repressor and activator of transcription (4). Research studies suggest this protein may function as either a tumor suppressor or an oncogene depending on tumor type, with up-regulation in human squamous cell carcinoma of the head and neck and down-regulation in colorectal carcinoma (5,6). The in vitro reprogramming of somatic cells to an embryonic-like state has been achieved by retroviral transduction of four factors: Oct-3/4, Sox2, c-Myc, and KLF4 (7). These induced pluripotent stem cells (iPS) are of great therapeutic interest as they exhibit the key characteristics and growth properties of pluripotent stem cells (8,9).

1. Yet, S.F. et al. (1998) J Biol Chem 273, 1026-31.
2. Segre, J.A. et al. (1999) Nat Genet 22, 356-60.
3. Katz, J.P. et al. (2005) Gastroenterology 128, 935-45.
4. Evans, P.M. and Liu, C. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 554-64.
5. Foster, K.W. et al. (2005) Oncogene 24, 1491-500.
6. Rowland, B.D. and Peeper, D.S. (2006) Nat Rev Cancer 6, 11-23.
7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
8. Meissner, A. et al. (2007) Nat Biotechnol 25, 1177-81.
9. Park, I.H. et al. (2008) Nature 451, 141-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.