Tau (Tau46) Mouse mAb

• 产品编号：CST-4019S      品牌：CST       原厂货号：4019S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Tau(Tau46) Mouse mAb 鼠单抗识别内源性的tau总蛋白，也与280kD的MAP2有交叉反应。根据其氨基酸序列推断，Tau (Tau46)Mouse mAb 鼠单抗可能可以识别tau蛋白的六种亚型。该单克隆抗体由自然小牛tau蛋白和抗原决定簇映射到蛋白羧基端肽段免疫动物产生。Tau是微管相关蛋白异构物，促进和稳定微管装配，尤其是在轴突中。有六个异构体已经被识别，它们有不同的氨基末端插入序列和不同数量的羧基末端串联重复序列，tau蛋白可被ERK、GSK-3和CDK5在大约25个位点处被过度磷酸化（1-2）。Tau蛋白磷酸化可降低其与微管的结合能力。神经纤维缠结是阿尔茨海默氏症的一个主要标志，这些缠结是由过度磷酸化tau蛋白组成的成对螺旋细丝束。GSK-3或CDK5对Tau蛋白Ser396的磷酸化可以导致微管的破坏，特别是在阿尔茨海默氏症中。此外，Tau蛋白包涵体还在其它神经退行性疾病中被发现，这些疾病被统称为Tau蛋白病(1,3)。

1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

Tau (Tau46) Mouse mAb detects endogenous levels of total tau protein and also cross-reacts with MAP2 at 280kD. Tau (Tau46) Mouse mAb is predicted to detect all six isoforms of tau based on the amino acid sequence.

Source / Purification

Monoclonal antibody is produced by immunizing animals with native bovine tau and the epitope maps to the carboxy-terminus of the protein.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody