Phospho-Troponin I (Cardiac) (Ser23/24) Antibody

• 产品编号：CST-4004S      品牌：CST       原厂货号：4004S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Troponin I (Cardiac) (Ser23/24) Antibody检测仅在Ser23/24位点磷酸化的内源性心脏的Troponin I蛋白。该抗体不能与磷酸化的骨骼肌troponin I发生交叉反应。通过人工合成人源troponin I蛋白Ser23/24位点周围相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Troponin蛋白连同tropomyosin蛋白一起发挥作用，该蛋白作为一个分子开关，在细胞内Ca2+浓度变化的情况下调节肌肉收缩。Troponin蛋白有三个亚型组成：Ca2+结合亚基troponin C (TnC)、肌球蛋白结合亚基troponin T (TnT)和抑制亚基troponin I (TnI)(1)。在心脏的β-adrenergic刺激下，TnI (cardiac)的Ser23和Ser24位点是通过PKA 和PKC 磷酸化。这个磷酸化刺激TnC蛋白的调节区域的构象变化，减少TnI和TnC的联系，并且通过减少肌丝Ca2+敏感度从而减少TnC蛋白的Ca2+结合度(1-3)。

1. Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
2. Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
3. Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.

原厂资料：

Specificity / Sensitivity

Phospho-Troponin I (Cardiac) (Ser23/24) Antibody detects endogenous levels of cardiac troponin I only when phosphorylated at Ser23/24.This antibody does not cross-react with phosphorylated skeletal muscle troponin I.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser23/24 of human troponin I (cardiac). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3).

1. Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
2. Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
3. Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody