Streptavidin-HRP

• 产品编号：CST-3999S      品牌：CST       原厂货号：3999S
• 产品分类：蛋白质检测与分析 > 蛋白标记和检测 > 亲和素、链霉亲和素、生物素 > 链霉亲和素
• 应用分类：

描述：

链霉亲和素与它的配体具有显著的高亲和力。生物素结合位点的复杂性和结构的不规则性使得它最适合与生物素结合并且表现出与链霉素亲和素-生物素复合体的高度特异性（4）。本产品用来检测生物素化的蛋白（1,2）。通过在链霉素亲和素的氨基基团交联辣根过氧化物酶（HRP）制成辣根过氧化物酶（HRP）和链霉素亲和素的结合体。链霉亲和素是从应用于生物分离和检测系统中链霉亲生物素蛋白中分离出来的53KDa同源四聚体（3），每一个链霉素亲和素的亚基可以与维生素形成高亲和力非共价结合。因为它的强高亲和力非共价结合，链霉素亲和素可以用于检测和分离生物素化的蛋白。化学反光检测系统已经成为western blots和ELISA检测中最好和最全面的检测方法。它们减少了放射性物质和有毒显色基质相关的危害。这些方法的检测速度和灵敏度的都是传统替代品无可比拟的。链霉素亲和素-HRP可以与生物素化的蛋白或者化学发光底物结合生成光信号。链霉素亲和素-HRP具有非常高的周转率，在较短的时间里有很高的敏感性。

原厂资料：

Specificity / Sensitivity

Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).

Source / Purification

Streptavidin-HRP is purified by gel filtration. The conjugate product is free from unconjugated streptavidin and HRP.

Description

This Cell Signaling Technology product is useful for the detection of biotinylated proteins (1,2). Conjugation of horseradish peroxidase (HRP) to streptavidin is obtained by cross linking the amino groups on streptavidin with the carbohydrate groups on HRP.

Background

Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins (1,2). 
 Chemiluminescent detection systems have emerged as the best all-around detection method for use with western blots and ELISA. These detection assays eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Streptavidin-HRP is used with biotinylated proteins and specific chemiluminescent substrates to generate light signal. Streptavidin-HRP conjugates have a very high turnover rate, coupling high sensitivity with short reaction times.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, 50% glycerol.
Store at -20ºC. Do not aliquot antibody.