IDH1 Antibody

• 产品编号：CST-3997S      品牌：CST       原厂货号：3997S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IDH1 Antibody用于检测内源性IDH1总蛋白水平，该抗体不与IDH2交互反应。多克隆抗体通过用对应于人源IDH1蛋白N端序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化。IDH1是三种异柠檬酸脱氢酶中的的一种，它负责催化异柠檬酸氧化脱羧生成α－酮戊二酸（α－KG）。该酶存在三个不同的亚类，它们分别利用NAD 和NADP+作为电子受体。IDH1 是NADP+依赖型的胞质和过氧化物酶体中的异柠檬酸脱氢酶。 IDH2 和3位于线粒体中，主要在三羧酸循环中发挥作用。IDH1在Ser103磷酸化后失活，它包含一个由两个多肽共同组成的钳状的结构域（2,3）。IDH1在大多数物种中甚至没有柠檬酸循环的生物中也有表达。最近，在恶性胶质瘤中发现了一种IDH1突变形式（4）。IDH1是一个肿瘤抑制因子，当它被突变后，会导致肿瘤新生，其中一部分的机制是通过诱导激活HIF-1通路（5）。

1. Ramachandran, N. and Colman, R.F. (1980) J Biol Chem 255, 8859-64.
2. Bennett, P.M. and Holms, W.H. (1975) J Gen Microbiol 87, 37-51.
3. Hurley, J.H. et al. (1990) Science 249, 1012-6.
4. Bleeker, F.E. et al. (2009) Hum Mutat 30, 7-11.
5. Zhao, S. et al. (2009) Science 324, 261-5.

原厂资料：

Specificity / Sensitivity

IDH1 Antibody detects endogenous levels of total IDH1 protein. The antibody does not cross react with IDH2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the N terminus of human IDH1. Antibodies were purified by protein A and peptide affinity chromatography.

Background

IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1). IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3). IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma (4). IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (5).

1. Ramachandran, N. and Colman, R.F. (1980) J Biol Chem 255, 8859-64.
2. Bennett, P.M. and Holms, W.H. (1975) J Gen Microbiol 87, 37-51.
3. Hurley, J.H. et al. (1990) Science 249, 1012-6.
4. Bleeker, F.E. et al. (2009) Hum Mutat 30, 7-11.
5. Zhao, S. et al. (2009) Science 324, 261-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.