Gα (pan) Antibody

• 产品编号：CST-3992S      品牌：CST       原厂货号：3992S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Gα(pan)抗体识别内源性的全部Gα蛋白。此抗体可能不识别G(12)-α, G(13)-α(13), G(s)-α(s)和G(olf)-α。该多克隆抗体用与人类鸟嘌呤核苷酸结合蛋白Gα(q)亚单位的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。异三聚鸟嘌呤核苷酸结合蛋白（G蛋白）由α、β和γ亚基构成，介导激素、神经递质、趋化因子和感觉刺激的作用。到今天为止，根据结构和功能的相似性，超过20个亚基分为四个家族，分别是Gα(s), Gα(i/o), Gα(q)和Gα(12)（1,2）。Gα(q)/Gα(11)的Tyr356磷酸化对G蛋白激活是至关重要的，因为苯丙氨酸替代Tyr356改变了Gα与受体的相互作用，并消除了配体诱导的IP3的形成（3）。

1. Offermanns, S. (2001) Oncogene 20, 1635-42.
2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
3. Umemori, H. et al. (1997) Science 276, 1878-81

原厂资料：

Specificity / Sensitivity

Gα (pan) Antibody detects total levels of endogenous G protein alpha subunits. The antibody may not detect G(12)-α, G(13)-α (13), G(s)-α (s) and G(olf)-α.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human guanine nucleotide-binding protein Gα (q) subunit. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).

1. Offermanns, S. (2001) Oncogene 20, 1635-42.
2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
3. Umemori, H. et al. (1997) Science 276, 1878-81

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.