GRK2 Antibody

• 产品编号：CST-3982S      品牌：CST       原厂货号：3982S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GRK2 Antibody 兔多抗识别内源性的总GRK2蛋白。该抗体也能识别GRK3，但与其它GRKs没有交叉反应。该多克隆抗体由合成的人类GRK2中氨基末端区域附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。G-蛋白偶联受体激酶2（GRK2），又称β-肾上腺素受体激酶1（β-ARK1），是GRK家族的成员，可以磷酸化活化的G蛋白偶联受体（GPCRs），启动G蛋白偶联受体的脱敏过程（1）。GRK2的激酶活性和细胞定位受到激活形式的受体、G-β和G-γ亚基、接头蛋白、磷脂、caveolin、钙调蛋白，以及磷酸化的严格调控（1）。PKC磷酸化促进其膜定位和取消抑制钙调蛋白关联，从而增强了GRK2的活动（2,3）。PKA磷酸化GRK2的Ser685，这有利于其与β-肾上腺素受体的结合（4）。ERK通过磷酸化Ser670抑制GRK2的活性（5）。Src磷酸化GRK2的多个酪氨酸残基（Tyr13，86和92），激活GRK2，促进GRK2的降解（6,7）。

1. Pitcher, J. A. et al. (1998) Annu. Rev. Biochem. 67, 653-692.
2. Winstel, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 2105-2109.
3. Krasel, C. et al. (2001) J. Biol. Chem. 276, 1911-1915.
4. Cong, M. et al. (2001) J. Biol. Chem. 276, 15192-15199.
5. Pitcher, J. A. et al. (1999) J. Biol. Chem. 274, 34531-34534.
6. Sarnago, S. et al. (1999) J. Biol. Chem. 274, 34411-34416.
7. Penela, P. et al. (2001) EMBO J. 20, 5129-5138.

原厂资料：

Specificity / Sensitivity

GRK2 Antibody detects endogenous levels of total GRK2. This antibody may also detect GRK3, but does not cross-react with other GRKs.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human GRK2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

G-protein-coupled receptor kinase 2 (GRK2), also known as beta-adrenergic receptor kinase 1 (beta-ARK1), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin and calmodulin, as well as by phosphorylation (1). PKC phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin (2,3). PKA phosphorylates GRK2 at Ser685, which facilitates the association of GRK2 with a beta-adrenergic receptor (4). Erk inhibits GRK2 activity via phosphorylation at Ser670 (5). Src phosphorylates GRK2 at multiple tyrosine residues (Tyr13, 86 and 92), which activates GRK2 activity and promotes GRK2 degradation (6,7).

1. Pitcher, J. A. et al. (1998) Annu. Rev. Biochem. 67, 653-692.
2. Winstel, R. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 2105-2109.
3. Krasel, C. et al. (2001) J. Biol. Chem. 276, 1911-1915.
4. Cong, M. et al. (2001) J. Biol. Chem. 276, 15192-15199.
5. Pitcher, J. A. et al. (1999) J. Biol. Chem. 274, 34531-34534.
6. Sarnago, S. et al. (1999) J. Biol. Chem. 274, 34411-34416.
7. Penela, P. et al. (2001) EMBO J. 20, 5129-5138.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody