CDX2 Antibody

• 产品编号：CST-3977S      品牌：CST       原厂货号：3977S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cdx2抗体识别内源性的总Cdx2蛋白.多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Cdx2蛋白的氨基末端氨基酸残基序列一致。抗体由肽段由蛋白A和亲和层析技术纯化.Cdx2, 同源异形盒结构域包含转录因子，是主要的滋养外胚层调控因子，这一层在哺乳动物发育中引起额外的胚胎组织（1）。Cdx2也涉及肠道发育（2）。获得表达或表达丧失与多种人类恶性肿瘤有关，如Barret Esophagus和结肠癌（4，5）。缺失Cdx2的小鼠胚胎干细胞显示有限的造血祖细胞发育和Hox基因表达变化（6）。CDX2也被认为是人AML细胞系中Hox基因表达异常的原因（7）。

1. Ralston, A. and Rossant, J. (2008) Dev Biol 313, 614-29.
2. Traber, P.G. and Silberg, D.G. (1996) Annu Rev Physiol 58, 275-97.
3. Shi, X.Y. et al. (2008) Am J Clin Pathol 129, 571-7.
4. Bakaris, S. et al. (2008) Histol Histopathol 23, 1043-7.
5. Guo, R.J. et al. (2004) Cancer Biol Ther 3, 593-601.
6. Wang, Y. et al. (2008) Proc Natl Acad Sci U S A 105, 7756-61.
7. Fröhling, S. et al. (2007) Cell Cycle 6, 2241-5.

原厂资料：

Specificity / Sensitivity

Cdx2 Antibody detects endogenous levels of total Cdx2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the N-terminus of human Cdx2. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

Cdx2, a homeobox domain-containing transcription factor, is a master regulator of the trophoectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development (1). Cdx2 is also involved in intestinal development (2), and gain of expression or loss of expression has been associated with various human malignancies such as Barret Esophagus (3) and colorectal cancer (4,5). Mouse embryonic stem cells deficient in Cdx2 display limited hematopoietic progenitor development and altered Hox gene expression (6), pointing to a role for Cdx2 in Hox gene regulation. Cdx2 is also implicated in the aberrant expression of Hox genes in human AML cell lines (7).

1. Ralston, A. and Rossant, J. (2008) Dev Biol 313, 614-29.
2. Traber, P.G. and Silberg, D.G. (1996) Annu Rev Physiol 58, 275-97.
3. Shi, X.Y. et al. (2008) Am J Clin Pathol 129, 571-7.
4. Bakaris, S. et al. (2008) Histol Histopathol 23, 1043-7.
5. Guo, R.J. et al. (2004) Cancer Biol Ther 3, 593-601.
6. Wang, Y. et al. (2008) Proc Natl Acad Sci U S A 105, 7756-61.
7. Fröhling, S. et al. (2007) Cell Cycle 6, 2241-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.