TRRAP (D2966) Antibody

• 产品编号：CST-3966S      品牌：CST       原厂货号：3966S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TRRAP (D2966) Antibody能够检测内源性TRRAP总蛋白水平。通过合成的与人源TRRAP蛋白相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Transformation/transcription domain-associated protein (TRRAP)是一个高度保守的434 kDa大小蛋白，该蛋白被发现在多种蛋白复合物中例如SAGA、PCAF、NuA4和TIP60，这些包含组蛋白乙酰转移酶(HAT)活性(1-4)。TRRAP功能作为一个接头蛋白，该蛋白通过直接结合到转录激活因子的转录激活区以及有助于HAT复合物的招募从而去使组蛋白乙酰化和激活转录(1-5)。TRRAP对于转录激活和c-Myc、E2F1、E2F4、p53以及腺病毒E1A蛋白的细胞转化激活是需要的(1,6,7)。TRRAP也是在早期发育期间有重要作用，并且在有丝分裂的检查点和正常细胞周期进程是需要的(8,9)。此外，研究显示TRRAP蛋白功能是在DNA修复起作用。作为TIP60复合物的部分，TRRAP对于在双链DNA中断和随后相应的重组的DNA修复中的histone H4的乙酰化是需要的(10)。此外，TRRAP与MRN (MRE11、RAD50、NBS1)复合物有关联，这缺少内部HAT活性，但是其功能在敏感性和随后双链中断的修复，该修复通过非同源DNA末端连接(11)。 TRRAP显示与ATM激酶家族的PI-3激酶区域有重要意义的同源性；然而，该激酶结构域的催化位点的氨基酸在TRRAP蛋白是不保守的(1)。

1. McMahon, S.B. et al. (1998) Cell 94, 363-374.
2. Grant, P.A. et al. (1998) Mol. Cell 2, 863-867.
3. Ikura, T. et al. (2000) Cell 102, 463-473.
4. Allard, S. et al. (1999) EMBO J. 18, 5108-5119.
5. McMahon, S.B. et al. (2000) Mol. Cell. Biol. 20, 556-562.
6. Deleu, L. et al. (2001) Oncogene 20, 8270-8275.
7. Ard, P.G. et al. (2002) Mol. Cell. Biol. 22, 5650-5661.
8. Herceg, Z. et al. (2001) Nat. Genet. 29, 206-211.
9. Li, H. et al. (2004) EMBO J. 23, 4824-4834.
10. Murr, R. et al. (2006) Nat. Cell Biol. 8, 91-99.
11. Robert, F. et al. (2006) Mol. Cell. Biol. 26, 402-412.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine, Horse

Specificity / Sensitivity

TRRAP (D2966) Antibody detects endogenous levels of total TRRAP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human TRRAP protein. Antibodies are purified by peptide affinity chromatography.

Background

Transformation/transcription domain-associated protein (TRRAP) is a highly conserved 434 kDa protein found in various multiprotein complexes, such as SAGA, PCAF, NuA4 and TIP60, which contain histone acetyltransferase (HAT) activity (1-4). TRRAP functions as an adaptor protein by binding directly to the transactivation domains of transcriptional activator proteins and facilitating the recruitment of HAT complexes to acetylate histone proteins and activate transcription (1-5). TRRAP is required for the transcriptional activation and cell transformation activities of c-Myc, E2F1, E2F4, p53 and the adenovirus E1A proteins (1,6,7). TRRAP is also essential in early development and is required at the mitotic checkpoint and for normal cell cycle progression (8,9). In addition, TRRAP has been shown to function in DNA repair. As part of the TIP60 complex, TRRAP is required for the acetylation of histone H4 at double-stranded DNA breaks and subsequent DNA repair by homologous recombination (10). In addition, TRRAP associates with the MRN (MRE11, RAD50, NBS1) complex, which lacks intrinsic HAT activity yet functions in the sensing and subsequent repair of double-stranded breaks by non-homologous DNA end-joining (11). TRRAP shows significant homology to the PI-3 kinase domain of the ATM family of kinases; however, amino acids that map to the catalytic site of the kinase domain are not conserved in TRRAP (1).

1. McMahon, S.B. et al. (1998) Cell 94, 363-374.
2. Grant, P.A. et al. (1998) Mol. Cell 2, 863-867.
3. Ikura, T. et al. (2000) Cell 102, 463-473.
4. Allard, S. et al. (1999) EMBO J. 18, 5108-5119.
5. McMahon, S.B. et al. (2000) Mol. Cell. Biol. 20, 556-562.
6. Deleu, L. et al. (2001) Oncogene 20, 8270-8275.
7. Ard, P.G. et al. (2002) Mol. Cell. Biol. 22, 5650-5661.
8. Herceg, Z. et al. (2001) Nat. Genet. 29, 206-211.
9. Li, H. et al. (2004) EMBO J. 23, 4824-4834.
10. Murr, R. et al. (2006) Nat. Cell Biol. 8, 91-99.
11. Robert, F. et al. (2006) Mol. Cell. Biol. 26, 402-412.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.