Ras Antibody

• 产品编号：CST-3965S      品牌：CST       原厂货号：3965S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Ras Antibody兔多抗能检测内源性总K-Ras, H-Ras和N-Ras蛋白水平。该抗体可能与R-Ras、 M-Ras发生交叉反应。该多克隆抗体通过使用与人源K-Ras蛋白胺端周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。21kDa的鸟苷酸结合蛋白(K-Ras, H-Ras, and N-Ras)在活化状态(GTP-bound)和失活状态(GDP-bound)不断转换（1）。受体酪氨酸激酶和G-蛋白偶联受体能够激活Ras，随后刺激Raf-MEK-MAPK信号通路(2-4)。GAP蛋白通常能够促进Ras的失活。但是，有研究表明，在30%的人类肿瘤中，Ras的点突变会阻止GAP介导的对该条通路的抑制作用（5）。肿瘤中最常见的致癌性Ras突变是Gly12突变为Asp（G12D），这会抑制Ras的失活，可能是通过增加蛋白的刚性而实现的(5,6）。

1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-54.
2. Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83.
3. Buday, L. and Downward, J. (1993) Cell 73, 611-20.
4. Huang, D.C. et al. (1993) Mol Cell Biol 13, 2420-31.
5. Bos, J.L. (1989) Cancer Res 49, 4682-9.
6. Ma, J. and Karplus, M. (1997) J Mol Biol 274, 114-31.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Chicken, Xenopus, Zebrafish, Dog

Specificity / Sensitivity

Ras Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras. This antibody may also cross-react with R-Ras and M-Ras.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues close to the amino-terminus of human K-Ras. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-54.
2. Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83.
3. Buday, L. and Downward, J. (1993) Cell 73, 611-20.
4. Huang, D.C. et al. (1993) Mol Cell Biol 13, 2420-31.
5. Bos, J.L. (1989) Cancer Res 49, 4682-9.
6. Ma, J. and Karplus, M. (1997) J Mol Biol 274, 114-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.